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| Status |
Public on Aug 20, 2023 |
| Title |
High-throughput Single-cell Profiling of B cell Responses Following Inactivated Influenza Vaccination in Young and Older Adults (single-cell Gene Expression) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Seasonal influenza contributes to a substantial disease burden annually, resulting in approximately 10 million hospital visits and 50 thousand deaths in a typical year in the US. 90% of the annual mortality from influenza occurs in people over the age of 65. While influenza vaccination is the best protection against the virus, it is less effective for the elderly. This may be due to differences in the quantity or type of B cells induced by vaccination in older individuals. To investigate this possibility, we leveraged recent development in single-cell technology that allows for simultaneous measurement of both gene expression profile and the B cell receptor (BCR) at single-cell resolution. Pre- and post-vaccination peripheral blood B cells were sorted from three young and three older adults who responded to the inactivated influenza vaccine and were profiled using single-cell RNAseq with paired BCR sequencing. At pre-vaccination, we observed a higher somatic hypermutation frequency and a higher abundance of activated B cells in older adults than in young adults. Following vaccination, young adults mounted a more clonal response than older adults. The response involved a mix of plasmablasts, activated B cells, and resting memory B cells in both age groups. The response in young adults was dominated by expansion in plasmablasts, while the response in older adults also involved activated B cells. We observed a consistent change in gene expression in plasmablasts after vaccination between age groups but not in the activated B cells. These quantitative and qualitative differences in the B cell response may provide insights into the age-related change of influenza vaccination response.
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| Overall design |
To understand the age-group difference in B cells underlying a successful vaccination response, we selected three young (20- to 30-years-old) and three older (60- to 100-years-old) subjects who responded to seasonal influenza vaccine with at least 4 fold increase of HAI titer for at least one vaccine strain from a cohort recruited at Yale University over the five vaccination seasons between 2010 and 2014.
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| Contributor(s) |
Melillo T, Wang M, Meng H, Kleinstein SH, Shaw A |
| Citation(s) |
37367734 |
| BioProject |
PRJNA728050 |
| |
| Submission date |
May 25, 2021 |
| Last update date |
May 10, 2024 |
| Contact name |
Steven Kleinstein |
| Organization name |
Yale University
|
| Department |
Pathology
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| Lab |
Kleinstein
|
| Street address |
300 George St.
|
| City |
New Haven |
| State/province |
Ct |
| ZIP/Postal code |
06511 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE175524 |
High-throughput Single-cell Profiling of B cell Responses Following Inactivated Influenza Vaccination in Young and Older Adults |
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