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| Status |
Public on May 24, 2024 |
| Title |
Blood DNA methylation altered by Mycobacterium tuberculosis infection in mice |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Knowing when a person was infected with Mycobacterium tuberculosis (M.tb) is critical as recent infection is the strongest clinical risk factor for progression to TB disease in immunocompetent individuals. However, time since M.tb infection is very difficult to determine in routine clinical practice. We determined whether DNA methylation patterns in the blood correlate with time since M.tb infection or exposure to develop a biomarker for recent exposure. As proof of concept, we found a DNA methylation signature that is present during early infection of mice that persists for at least 5 months post-infection.
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| Overall design |
Blood was collected from 54 C57BL/6 mice and 46 BALB/c mice infected with a low dose of M.tb (Erdman), as well as 23 C57BL/6 mice and 28 BALB/c mice that served as uninfected, age-matched controls. Blood was collected at days 7, 14, 30, 62, 90, 120 and 153 post-infection. Whole blood was subjected to reduced representation bisulfite sequencing. We identify stable DNA methylation changes associated with infection.
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| Contributor(s) |
Ault RC, Headley CA, Piergallini TJ, Gautam S, Dwivedi V, Carless MA, Turner J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 27, 2021 |
| Last update date |
May 24, 2024 |
| Contact name |
Russell Ault |
| Organization name |
The Ohio State University College of Medicine
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| Street address |
370 W 9th Ave
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| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (151)
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| Relations |
| BioProject |
PRJNA733167 |
| SRA |
SRP321639 |
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