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| Status |
Public on Aug 03, 2021 |
| Title |
Transcriptome analysis of total RNA from Rhodobacter sphaeroides 2.4.1 wildtype and StsR (RSs0827) deletion strain during different growth phases |
| Organism |
Cereibacter sphaeroides 2.4.1 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The sRNA StsR (formerly RSs0827) was first described as an sRNA induced upon iron starvation and was later demonstrated to be the most highly induced RNA in late stationary phase in the phototrophic Alphaproteobacterium R. sphaeroides. StsR is under control of RpoHI/RpoHII and therefore induced by multiple stress factors. To investigate the function of this sRNA on the hole transcriptome of R. sphaeroides we performed RNAseq with cultures of the exponential growth (6 h) phase, stationary growth phase (72 h / 144 h) and outgrowth phase. Cultures were grown under microaerobic growth conditions in biological triplicates.
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| Overall design |
This data includes the transcriptome data of two different strains each in triplicates: Rhodobacter sphaeroides 2.4.1 (wildtype) and R. sphaeroides 2.4.1 StsR deletion strain (d0827). Total RNA of all strains was isolated and sequenced.
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| Contributor(s) |
Eisenhardt K, Förstner K, Klug G |
| Citation(s) |
34299177 |
| |
| Submission date |
Jun 02, 2021 |
| Last update date |
Aug 03, 2021 |
| Contact name |
Gabriele Klug |
| E-mail(s) |
Gabriele.Klug@mikro.bio.uni-giessen.de
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| Organization name |
Justus-Liebig University Gießen
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| Department |
Molecular- and Microbiology
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| Street address |
Heinrich-Buff-Ring 26-32
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| City |
Gießen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
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| Platforms (1) |
| GPL28141 |
Illumina NextSeq 500 (Rhodobacter sphaeroides 2.4.1) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA734528 |
| SRA |
SRP322336 |
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