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Series GSE17605 Query DataSets for GSE17605
Status Public on Jan 06, 2010
Title Xylella fastidiosa 9a5c: gomesin-treated cells vs. control
Organism Xylella fastidiosa
Experiment type Expression profiling by array
Summary In the xylem vessels of susceptible hosts, such as citrus trees or grapevines, Xylella fastidiosa forms biofilm like-colonies that can block water transport, which appears to correlate to disease symptoms. Besides helping host colonization, bacterial biofilms play an important role in resistance against antimicrobial agents, for instance antimicrobial peptides (AMP). Here we show that gomesin, a potent AMP from a Brazilian tarantula spider, modulates X. fastidiosa gene expression profile upon 60 min treatment with a sublethal concentration. Data from DNA microarray hybridizations revealed that among the up-regulated coding sequences (CDS), some are related to biofilm production. In addition, we show that the biofilm formed by gomesin-treated bacteria is thicker than that formed by non-treated cells or cells exposed to streptomycin. We have also observed that treatment of X. fastidiosa with sublethal concentration of gomesin before inoculation in tobacco plants correlates with reduction in CVC symptoms, an effect possibly due to trapping of bacterial cells to fewer xylem vessels given the enhancement in biofilm production. Together, our results suggest that X. fastidiosa can selectively sense a sublethal concentration of gomesin modulating its gene expression to produce a stronger biofilm that may protect itself against the toxic effects of this AMP.
Overall design Two-condition experiment, control (PW media) vs. gomesin-treated cells. Biological replicates: 5 control, 5 gomesin-treated, independently grown and harvested. Two replicates (left and right side of slide) per array. Total of 10 slides were hibridized, including dye-swap of and two self-self experiments.
Contributor(s) Fogaça AC, Zaini PA
Citation(s) 20370836
Submission date Aug 12, 2009
Last update date Mar 21, 2012
Contact name Paulo A. Zaini
Organization name Universidade de São Paulo
Department Bioquímica
Lab Biologia Molecular de Microorganismos
Street address Av. Prof. Lineu Prestes, 748 - Butantã
City São Paulo
State/province São Paulo
ZIP/Postal code 05508-900
Country Brazil
Platforms (1)
GPL9024 IQ-USP Xylella fastidiosa 9.2K v2.0.
Samples (20)
GSM439466 control vs. gomesin-treated for 60 minutes. Replica 1
GSM439467 control vs. gomesin-treated for 60 minutes. Replica 2
GSM439468 control vs. gomesin-treated for 60 minutes. Replica 3
BioProject PRJNA118559

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17605_RAW.tar 3.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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