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Series GSE176069 Query DataSets for GSE176069
Status Public on Jul 26, 2021
Title In vitro differentiation of hepatoblasts into hepatocytes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We differentiated primary mouse E14.5 Dlk1+ hepatoblasts in vitro into hepatocyte-like cells that recapitulated morphological features of hepatocytes. To assess the level of differentiation, we performed RNA-seq analysis and compared gene expression profiles of hepatobalsts, in vitro differentiated hepatocytes and mature hepatocytes isolated from adult mouse livers as a control.
 
Overall design Hepatoblasts, in vitro differetniated hepatocytes and mature hepatocytes were collected in 4 biological replicates. Hepatoblasts were isolated based on the Dlk1 surface marker using MACS (Tanimizu et al., 2003, JCS). The isolated cells were cultured on fibronectin coated plates in optimized Differentiation media for 4 days (Belicova et al., 2021, JCB). The adult mouse liver hepatocytes were isolated according to a published colagenase perfusion protocol (Klingmüller et al., 2006, IEE Proceedings: Systems Biology).
 
Contributor(s) Belicova L, Räägel H
Citation(s) 34328499
Submission date Jun 03, 2021
Last update date Aug 11, 2021
Contact name Lenka Belicova
Organization name MPI-CBG Dresden
Street address Pfotenhauerstr. 108
City Dresden
State/province Saxony
ZIP/Postal code 01309
Country Germany
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (12)
GSM5354402 Hepatoblasts_rep1
GSM5354403 Hepatoblasts_rep2
GSM5354404 Hepatoblasts_rep3
Relations
BioProject PRJNA734790
SRA SRP322417

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE176069_raw_counts_matrix.txt.gz 572.9 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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