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| Status |
Public on Jun 04, 2021 |
| Title |
Expression data of extracellular vesicles from bone metastatic breast cancer cell line |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Bone metastasis is an incurable complication of breast cancer. In advanced stages, patients with estrogen-positive tumors experience significantly higher incidence of bone metastasis compared to estrogen-negative patients. To investigate how estrogen-positive breast cancer cells communicate with bone microenvironmental cells, extracellular vesicles from bone metastatic MCF7BoM2 were purified and analyzed.
We used GeneChip miRNA 4.1 Array to detail the miRNA expression in the extracellular vesicles from the bone metastatic MCF7BoM2 and its parental cell line MCF7, and identified the dysregulated miRNAs.
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| Overall design |
Extracellular vesicles from cells were isolated by ultracentrifugation. Cells were grown in extracellular vesicles-depleted media for 48 h before conditioned media were collected for extracellular vesicles purification. To isolate total extracellular vesicles, conditioned media was centrifuged at 300g for 10 minutes to remove cells. Extracellular vesicles were collected by ultracentrifugation at 120,000g for 70 minutes, folloed by RNA extraction and hybridization on GeneChip miRNA 4.1 Array.
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| Contributor(s) |
Wu K, Wu S, Zhao D, Tyagi A, Pramod Deshpande R, Watabe K |
| Citation(s) |
34465793 |
| Submission date |
Jun 03, 2021 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Kounosuke Watabe |
| Organization name |
Wake Forest University School of Medicine
|
| Street address |
1 Medical Center Blvd
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| City |
Winston-Salem |
| State/province |
NC |
| ZIP/Postal code |
27157 |
| Country |
USA |
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| Platforms (1) |
| GPL21572 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA734919 |
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