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| Status |
Public on Jun 06, 2021 |
| Title |
RNA- and ATAC-seq data from hESCs differentiated with Wnt and Activin with or without CPI-203 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report genome-wide changes in chromatin state and transcriptional output following Wnt-priming and subsequent Activin induced differentiation of human embryonic stem cell and the effects of BET bromodomain inhibition during Wnt-priming. The two signals, Wnt and Activin, although provided sequentially, result in stable mesendoderm differentiation (Yoney et al., 2018).
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| Overall design |
We performed RNA-seq and ATAC-seq of human embryonic stem cells differentiated with Wnt and Activin and treated with BET inhibitor, CPI-203 (Selleckchem, Cat. No. S7304). Two replicates per condition for both RNA- and ATAC-seq were performed and samples were collected after 24 or 48 h.
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| Contributor(s) |
Yoney A, Bai L, Brivanlou AH, Siggia ED |
| Citation(s) |
35815787 |
| |
| Submission date |
Jun 05, 2021 |
| Last update date |
Sep 14, 2022 |
| Contact name |
Anna Yoney |
| E-mail(s) |
ay2520@cumc.columbia.edu
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| Organization name |
Columbia University
|
| Department |
Genetics and Development
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| Street address |
701 W 168th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA735402 |
| SRA |
SRP322871 |
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