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Series GSE176299 Query DataSets for GSE176299
Status Public on Jun 08, 2021
Title Mild food restriction and gene expression profile (muscle)
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The aim of this study is to obtain the gene expression profiles of the muscle of young growing rats after mild restriction of food intake for one week or one month.
 
Overall design Male Wistar rats, at 6 weeks of age, were fed with reduced diet (5%, 10%, 20% and 30% restriction) for one week or one month (n=5). After 16h fasting, the muscle was then extracted and total RNA was isolated using an RNeasy mini kit with DNase treatment (QIAGEN). The RNA samples were pooled from five rats for each group. Then, DNA microarray analysis was performed using the GeneChip (Affymetrix) on each pooled RNA sample.
 
Contributor(s) Saito K, Kato H
Citation(s) 34209243
Submission date Jun 07, 2021
Last update date Aug 03, 2021
Contact name Kenji Saito
E-mail(s) kkkj774@yahoo.co.jp
Phone +81-3-5841-5114
Organization name The Univeristy of Tokyo
Department Department of Applied Biological Chemistry
Lab Nutritional Biochemistry
Street address 1-1-1, yayoi
City bunkyo-ku
ZIP/Postal code 113-8657
Country Japan
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (10)
GSM5362380 muscle 1week control
GSM5362381 muscle 1week 5%CR
GSM5362382 muscle 1week 10%CR
This SubSeries is part of SuperSeries:
GSE176300 Mild food restriction and gene expression profile
Relations
BioProject PRJNA735743

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE176299_RAW.tar 23.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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