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| Status |
Public on Dec 01, 2009 |
| Title |
The human reticulocyte transcriptome (HG-U133_Plus2.0) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
RNA from circulating blood reticulocytes was utilized to provide a robust description of genes transcribed at the final stages of erythroblast maturation. After depletion of leukocytes and platelets, Affymetrix HG-U133Plus 2.0 arrays were hybridized with probe from total RNA isolated from blood sampled from 6 umbilical cords and 6 healthy adult humans.
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| Overall design |
Peripheral blood from 6 normal adults and 6 human umbilical cords were purified to obtain packed red blood cells and their purity was assessed by CELL-DYN4000. The contaminated WBC count was less than 1 out of 1 million cells. Total RNA from purified reticulocytes included in the purified red blood cells was extracted, labeled, and hybridized onto Affymetrix HG-U133Plus 2.0 arrays.
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| Contributor(s) |
Goh S, Miller JL |
| Citation(s) |
19939273 |
| Submission date |
Aug 13, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Jeffery Lynn Miller |
| E-mail(s) |
jm7f@nih.gov
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| Phone |
301-480-1908
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| Fax |
301-435-5148
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| Organization name |
NIH/NIDDK
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| Department |
Molecular Medicine Branch
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| Lab |
Molecular Biology and Genetics Section
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| Street address |
10 Center Drive
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| GSM440234 |
Adult Blood 15, biological replicate 1 |
| GSM440235 |
Adult Blood 16, biological replicate 2 |
| GSM440236 |
Adult Blood 17, biological replicate 3 |
| GSM440237 |
Adult Blood 18, biological replicate 4 |
| GSM440238 |
Adult Blood 19, biological replicate 5 |
| GSM440239 |
Adult Blood 20, biological replicate 6 |
| GSM440240 |
Cord Blood 15, biological replicate 1 |
| GSM440241 |
Cord Blood 16, biological replicate 2 |
| GSM440242 |
Cord Blood 17, biological replicate 3 |
| GSM440243 |
Cord Blood 18, biological replicate 4 |
| GSM440244 |
Cord Blood 19, biological replicate 5 |
| GSM440245 |
Cord Blood 20, biological replicate 6 |
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| Relations |
| BioProject |
PRJNA118581 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17639_RAW.tar |
103.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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