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Series GSE176445 Query DataSets for GSE176445
Status Public on Jan 07, 2022
Title Transciptional profiling of human mTEChi
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Aire-sensitive genes exhibit a weak inclusion of alternative splicing events
 
Overall design Thymi from cardiac surgery pediatric patients were minced in 1xPBS to release the thymocytes. Then treated four times with collagenase/dispase (1:200) and DNaseI (1:1000) digestion, and the cell fractions were further dissociated with gentleMACS treatment. All fractions were combined and filtered through the 100μm Falcon cell strainer. The cells were collected by centrifugation, washed, and transferred to Optiprep density gradient. The upper cell layer was combined into one tube, washed, and counted resulting in 0.4-6.5x 10^8 thymic cells. The cell fraction was then depleted with human CD45 beads (100ul beads per 2x10^8 cells; Miltenyi Biotec) using autoMACS. After the CD45 depletion, the cells were sorted with BD FACSAria for mTEC MHChi (CD45- CDR2- EpCAM+ HLA-DRhi) directly into TRIzol (ThermoFisher) solution.
 
Contributor(s) Peterson P, Giraud M
Citation(s) 35037357
Submission date Jun 09, 2021
Last update date Jan 24, 2022
Contact name Matthieu Giraud
E-mail(s) matthieu.giraud@inserm.fr
Organization name INSERM _ Université de Nantes
Department CRTI
Street address 30 bd Jean Monnet
City Nantes
ZIP/Postal code 44000
Country France
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (5)
GSM5365683 mTEChi indiv1
GSM5365684 mTEChi indiv2
GSM5365685 mTEChi indiv3
Relations
BioProject PRJNA736302
SRA SRP323341

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE176445_RAW.tar 13.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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