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Series GSE176464 Query DataSets for GSE176464
Status Public on Feb 04, 2022
Title Regulation of alternative splicing by a novel BRR2 interactor C9ORF78
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The RNA helicase BRR2 (SNRNP200) is one of the key remodeling factors of the spliceosome. Here we show its direct interaction with C9ORF78, a poorly characterized protein predicted to be largely intrinsically disordered. We present cryo-EM structures showing how C9ORF78 and the spliceosomal B-complex protein FBP21 wrap around the C-terminal helicase cassette of BRR2 and that binding of the two proteins is mutually exclusive. C9ORF78 associates with the spliceosome, as we confirm via proteomics and RNA UV-crosslinking. An siRNA mediated C9ORF78 knockdown reveals changes in alternative splicing of specific target pre-mRNAs, which in part depend on its interaction with BRR2. In particular, C9ORF78 regulates a substantial number of alternative 3’ splice sites, which might be facilitated through an additional interaction with human PRP22 (DHX8).
Overall design Identification of C9orf78 RNA targets with FLASH, using GFP as a negative control
Contributor(s) Ilik I, Bergfort A, Preußner M
Citation(s) 35241646
Submission date Jun 09, 2021
Last update date Mar 30, 2022
Organization name Max-Planck-Institut für molekulare Genetik
Lab OWL Aktas
Street address Ihnestraße 63-73
City Berlin
State/province Berlin
ZIP/Postal code 14195
Country Germany
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM5365951 C9orf78 rep1
GSM5365952 C9orf78 rep2
GSM5365953 GFP rep1
BioProject PRJNA736337
SRA SRP323369

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Supplementary file Size Download File type/resource
GSE176464_RAW.tar 33.0 Mb (http)(custom) TAR (of BW)
GSE176464_new_flash_barcodes.fa.gz 158 b (ftp)(http) FA
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Raw data are available in SRA
Processed data provided as supplementary file

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