 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 25, 2009 |
| Title |
Transcriptome comparison of Streptococcus pyogenes strain MGAS2221 with that of an isogenic PEL mutant |
| Organism |
Streptococcus pyogenes |
| Experiment type |
Expression profiling by array
|
| Summary |
PEL is a small regulatory RNA that is present within the S. pyogenes genome. In some strains of S. pyogenes, the PEL sRNA regulates many of the known virulence factors produced by this pathogen. A genome-wide analysis of PEL-regulated genes had not been performed previously so we analyzed this by comparing the parental M1T1 isolate MGAS2221 with an isogenic PEL mutant. Comparing gene transcript levels between these two strains at the exponential and stationary phases of growth, we identified that PEL has no regulatory function in MGAS2221.
|
| |
|
| Overall design |
Three cultures of each GAS strain were grown in THY broth to exponential (O.D. 0.5) and stationary (O.D. 1.7) phases of growth. Two volumes of RNA protect were added to samples recovered at these O.D.'s, the samples incubated at room temperature for 5 minutes, and the bacteria collected through centrifugation. Total RNA was isolated via a mechanical disruption method, converted to cDNA, fragmented, labeled, and hybridized to our Affymetrix microarray. Estimates of gene expression were calculated using GCOS software v1.4.
|
| |
|
| Contributor(s) |
Sumby P |
| Citation(s) |
19888332 |
| |
| Submission date |
Aug 22, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Paul Sumby |
| Organization name |
University of Nevada
|
| Department |
Microbiology & Immunology
|
| Street address |
1554 N. Virginia St.
|
| City |
Reno |
| State/province |
NV |
| ZIP/Postal code |
89557 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9080 |
TMHRI/Affy Streptococcus pyogenes array |
|
| Samples (12)
|
| GSM443784 |
Strain MGAS2221 in THY, exponential, sample A |
| GSM443785 |
Strain MGAS2221 in THY, exponential, sample B |
| GSM443786 |
Strain MGAS2221 in THY, exponential, sample C |
| GSM443787 |
Strain 2221ΔPEL in THY, exponential, sample A |
| GSM443788 |
Strain 2221ΔPEL in THY, exponential, sample B |
| GSM443789 |
Strain 2221ΔPEL in THY, exponential, sample C |
| GSM443790 |
Strain MGAS2221 in THY, stationary, sample A |
| GSM443791 |
Strain MGAS2221 in THY, stationary, sample B |
| GSM443792 |
Strain MGAS2221 in THY, stationary, sample C |
| GSM443793 |
Strain 2221ΔPEL in THY, stationary, sample A |
| GSM443794 |
Strain 2221ΔPEL in THY, stationary, sample B |
| GSM443795 |
Strain 2221ΔPEL in THY, stationary, sample C |
|
| This SubSeries is part of SuperSeries: |
| GSE17790 |
A genome-wide analysis of small regulatory RNAs in the human pathogen group A Streptococcus |
|
| Relations |
| BioProject |
PRJNA123283 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17767_RAW.tar |
82.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...