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| Status |
Public on Feb 28, 2010 |
| Title |
Iron Chelators Treatment on MCF-7 Human Breast Cancer Cell |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Iron-deficiency affects 500 million people, yet the molecular role of iron in gene expression remains poorly characterized. Moreover, the alterations in global gene expression after iron chelation remains unclear and are important to assess for understanding the molecular pathology of iron-deficiency and the biological effects of iron chelators. We assessed the effect on whole genome gene expression of two iron chelators (desferrioxamine and 2-hydroxy-1-napthylaldehyde isonicotinoyl hydrazone) that have markedly different permeability properties. Sixteen genes were significantly regulated by both chelators, while a further 50 genes were regulated by either ligand. Most of the genes identified in this study have not been previously described to be iron-regulated and are important for understanding the molecular and cellular effects of iron-deficiency.
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| Overall design |
The MCF-7 cells were incubated with either control medium or this medium containing DFO (250 µM) or 311 (25 µM) for 24 h at 37oC. These concentrations were used since our previous studies demonstrated that under these conditions these chelators lead to up-regulation of iron-responsive genes such as the TfR1 after this incubation time.8 Moreover, the higher concentration of DFO was implemented due to its limited ability to permeate cell membranes.
Total RNA was isolated from cells in 1 mL of TRIzol® reagent (Invitrogen). Samples were then prepared and hybridized to the Human Genome U133 Plus 2.0 430 2.0. The human GeneChip® U133 Plus 2.0 consists of greater than 47,000 transcripts and variants from over 38,500 well characterized human genes. On completion of hybridization and washing, microarray chips were scanned with the Affymetrix GeneChip® Scanner 3000 (Affymetrix).
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| Contributor(s) |
Fu D, Yu Y, Saletta F, Suryo Rahmanto Y, Richardson DR |
| Citation(s) |
20023006 |
| Submission date |
Aug 27, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Des Raymond Richardson |
| E-mail(s) |
d.richardson@med.usyd.edu.au
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| Organization name |
University of Sydney
|
| Department |
Bosch Institute
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| Lab |
Pathology
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| Street address |
92-94 Parramatta Rd, Sydney
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| City |
Sydney |
| State/province |
New South Wales |
| ZIP/Postal code |
2006 |
| Country |
Australia |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (3) |
| GSM444834 |
MCF-7 Human Breast Cancer Cell Line - affs196-mem |
| GSM444835 |
MCF-7 Human Breast Cancer Cell Line - affs196-dfo |
| GSM444836 |
MCF-7 Human Breast Cancer Cell Line - affs196-311 |
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| Relations |
| BioProject |
PRJNA117911 |
