|
| Status |
Public on Sep 08, 2022 |
| Title |
Stem Cell transcriptional response to Microglia-Conditioned Media |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This paper demonstrates that whereas SOX2+ cells in HDB are highly susceptible to ZKV, SOX2+ primary GBM samples exhibit moderate to high resistance to infection. Expression profiling revealed an innate immune signature due to microglia infiltration in highly resistant GBM samples that was lacking in HDB. This data was generated to proflile the transcriptional response to microglia-secreted factors in stem cells from glioma cell lines and human developing brain. We find that Interferon-stimulated genes are the main targets upregulated in response to microglia conditioned media exposure.
|
| |
|
| Overall design |
GCGR glioma stem cell lines (G322, G334) and primary human developing brain cultures (BRC2251) were exposed to microglia-conditioned media (067,069,070) or to GSC-conditioned control media (G322, G334, BRC2251) media
|
| |
|
| Contributor(s) |
Bulstrode H, Moutsopoulos I, Mohorianu I, Rowitch D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 21, 2021 |
| Last update date |
Sep 08, 2022 |
| Contact name |
Irina Mohorianu |
| E-mail(s) |
data-submissions@stemcells.cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Wellcome-MRC Cambridge Stem Cell Institute
|
| Street address |
Puddicombe Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (34)
|
|
| This SubSeries is part of SuperSeries: |
| GSE178623 |
Microglia regulate Zika virus infection in human developing brain and glioma; Stem Cell transcriptional response to Microglia-Conditioned Media |
|
| Relations |
| BioProject |
PRJNA739735 |
| SRA |
SRP324925 |
Less...
More...