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Series GSE178620 Query DataSets for GSE178620
Status Public on Sep 08, 2022
Title Stem Cell transcriptional response to Microglia-Conditioned Media
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary This paper demonstrates that whereas SOX2+ cells in HDB are highly susceptible to ZKV, SOX2+ primary GBM samples exhibit moderate to high resistance to infection. Expression profiling revealed an innate immune signature due to microglia infiltration in highly resistant GBM samples that was lacking in HDB. This data was generated to proflile the transcriptional response to microglia-secreted factors in stem cells from glioma cell lines and human developing brain. We find that Interferon-stimulated genes are the main targets upregulated in response to microglia conditioned media exposure.
Overall design GCGR glioma stem cell lines (G322, G334) and primary human developing brain cultures (BRC2251) were exposed to microglia-conditioned media (067,069,070) or to GSC-conditioned control media (G322, G334, BRC2251) media
Contributor(s) Bulstrode H, Moutsopoulos I, Mohorianu I, Rowitch D
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Submission date Jun 21, 2021
Last update date Sep 08, 2022
Contact name Irina Mohorianu
Organization name University of Cambridge
Department Wellcome-MRC Cambridge Stem Cell Institute
Street address Puddicombe Way
City Cambridge
ZIP/Postal code CB2 0AW
Country United Kingdom
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (34)
GSM5394535 microglia_067MG_G322_1
GSM5394536 microglia_067MG_G322_2
GSM5394537 microglia_069MG_G322_1
This SubSeries is part of SuperSeries:
GSE178623 Microglia regulate Zika virus infection in human developing brain and glioma; Stem Cell transcriptional response to Microglia-Conditioned Media
BioProject PRJNA739735
SRA SRP324925

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE178620_normalised_abundances.csv.gz 2.8 Mb (ftp)(http) CSV
GSE178620_raw_abundances.csv.gz 1.8 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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