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Series GSE178661 Query DataSets for GSE178661
Status Public on Nov 25, 2022
Title Zygotic genome activation by the totipotency pioneer factor Nr5a2
Organism Mus musculus
Experiment type Expression profiling by array
Summary Life begins with a switch in genetic control from the maternal to the embryonic genome during zygotic genome activation (ZGA). Despite its importance, the essential regulators of ZGA remain largely unknown in mammals. Based on de novo motif searches, we identified the orphan nuclear receptor Nr5a2 as a key activator of major ZGA in mouse 2-cell embryos. Nr5a2 is required for progression beyond the 2-cell stage. It binds to its motif within SINE B1/Alu retrotransposable elements found in cis-regulatory regions of ZGA genes. Chemical inhibition suggests that 72% of ZGA genes are regulated by Nr5a2 and potentially other orphan nuclear receptors. Nr5a2 promotes chromatin accessibility during ZGA and binds nucleosomal DNA in vitro. We conclude that Nr5a2 is an essential pioneer factor that regulates ZGA.
 
Overall design RNA seq on small batches of MII eggs and early preimplantation mouse embryos in triplicates in WT C57BL/6i strain and in B6CAST F1 hybrids.
 
Citation(s) 36423263
Submission date Jun 22, 2021
Last update date Nov 25, 2022
Contact name Pavel Andreevich Kravchenko
E-mail(s) pkravchenko@biochem.mpg.de
Phone +4915110675513
Organization name Max-Planck-Institute of Biochemistry
Department Totipotency
Lab Tachibana
Street address Am Klopferspitz 18
City Munich
State/province Planegg
ZIP/Postal code 82152
Country Germany
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (398)
GSM5395097 Single cell RNA-Seq 72585 WT MIIegg
GSM5395098 Single cell RNA-Seq 72586 WT MIIegg
GSM5395099 Single cell RNA-Seq 72587 WT MIIegg
This SuperSeries is composed of the following SubSeries:
GSE178638 Zygotic genome activation by the totipotency pioneer factor Nr5a2 I
GSE178654 Zygotic genome activation by the totipotency pioneer factor Nr5a2 II
GSE178658 Zygotic genome activation by the totipotency pioneer factor Nr5a2 III
Relations
BioProject PRJNA739972

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