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| Status |
Public on Jul 09, 2021 |
| Title |
The WDR5-H3K4me3 epigenetic axis regulates OPN expression to compensate PD-L1 function to promote pancreatic cancer immune escape [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Total RNA was isolated from normal C57BL/6 mouse pancreas, PANC02-H7, and UN-KC-6141 tumors from tumor-bearing mice. The RNA was used for RNA-Seq. The gene expression profiles between normal mouse pancreas and orthotopic pancreatic tumors were compared, and differentially expressed genes were identified.
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| Overall design |
Normal mouse pancreas and tumors from tumor-bearing mice were used for chromatin fragment preparation. Anti-H3K4me3 antibody was used for ChIP, followed by library construction and ChIP-sequencing. The genome-wide H3K4me3 profiles between normal mouse pancreas and orthotopic pancreatic tumors were compared. Genes with differential H3K4me3 depositions at their promoter regions were identified.
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| Contributor(s) |
Shi H, Wu C, Liu K |
| Citation(s) |
34326167 |
| |
| Submission date |
Jun 22, 2021 |
| Last update date |
Aug 24, 2021 |
| Contact name |
Huidong Shi |
| E-mail(s) |
hshi@augusta.edu
|
| Phone |
706-721-6000
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| Organization name |
Augusta University
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| Department |
Georgia Cancer Center
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| Lab |
2125 K
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| Street address |
1120 15th Street, CN2138
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| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE178677 |
The WDR5-H3K4me3 epigenetic axis regulates OPN expression to compensate PD-L1 function to promote pancreatic cancer immune escape |
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| Relations |
| BioProject |
PRJNA740000 |
| SRA |
SRP325084 |
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