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| Status |
Public on Aug 14, 2010 |
| Title |
Global transcriptome profiling of wild soybean (Glycine soja) roots under NaHCO3 treatment |
| Platform organism |
Glycine max |
| Sample organism |
Glycine soja |
| Experiment type |
Expression profiling by array
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| Summary |
Plant roots are the primary site of perception and injury for saline-alkaline stress. The current knowledge of the saline-alkaline stress transcriptome is most focused on salt (NaCl) stress. Only a little alkaline (NaHCO3) stress transcriptome is limited to one time point after stress. Time-course analysis and comparative investigation on roots in the alkaline stress condition are needed to understand the gene response networks that are subject to alkaline tolerance.
We used microarrays to detail the global programme of gene expression underlying NaHCO3 treatment and identified distinct classes of regulated genes during this process.
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| Overall design |
Three week old Glycine soja seedling roots from 3cm root apex were harvested in two independent biological replicates after 0, 0.5, 1, 3, 6, 12 and 24h treatment with 50mmol/L NaHCO3 stress for RNA extraction and hybridization on Affymetrix microarrays. To minimize biological variance, roots from three plants originating from the same experiment, condition and cultivar was pooled.
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| Contributor(s) |
Ge Y, Li Y, Lv D, Zhu Y |
| Citation(s) |
20653984 |
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| Submission date |
Aug 31, 2009 |
| Last update date |
Nov 22, 2016 |
| Contact name |
Yanming Zhu |
| E-mail(s) |
ymzhu2001@neau.edu.cn
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| Phone |
86-451-55190734
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| Organization name |
Northeast Agriculture University
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| Department |
Life sciences
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| Lab |
Plant Bioengineering laboratory
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| Street address |
Wood Street 59
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| City |
Harbin |
| State/province |
Heilongjiang |
| ZIP/Postal code |
150030 |
| Country |
China |
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| Platforms (1) |
| GPL4592 |
[Soybean] Affymetrix Soybean Genome Array |
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| Samples (14)
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| GSM446674 |
Glycine soja, roots from 3cm root apex, standard condition, replicate 1 |
| GSM446675 |
Glycine soja, roots from 3cm root apex, standard condition, replicate 2 |
| GSM446676 |
Glycine soja, roots from 3cm root apex, 50 mM NaHCO3, 30 minutes, replicate 1 |
| GSM446677 |
Glycine soja, roots from 3cm root apex, 50 mM NaHCO3, 30 minutes, replicate 2 |
| GSM446678 |
Glycine soja, roots from 3cm root apex, 50 mM NaHCO3, 1 hour, replicate 1 |
| GSM446679 |
Glycine soja, roots from 3cm root apex, 50 mM NaHCO3, 1 hour, replicate 2 |
| GSM446680 |
Glycine soja, roots from 3cm root apex, 50 mM NaHCO3, 3 hour, replicate 1 |
| GSM446681 |
Glycine soja, roots from 3cm root apex, 50 mM NaHCO3, 3 hour, replicate 2 |
| GSM446682 |
Glycine soja, roots from 3cm root apex, 50 mM NaHCO3, 6 hour, replicate 1 |
| GSM446683 |
Glycine soja, roots from 3cm root apex, 50 mM NaHCO3, 6 hour, replicate 2 |
| GSM446684 |
Glycine soja, roots from 3cm root apex, 50 mM NaHCO3, 12 hour, replicate 1 |
| GSM446685 |
Glycine soja, roots from 3cm root apex, 50 mM NaHCO3, 12 hour, replicate 2 |
| GSM446686 |
Glycine soja, roots from 3cm root apex, 50 mM NaHCO3, 24 hour, replicate 1 |
| GSM446687 |
Glycine soja, roots from 3cm root apex, 50 mM NaHCO3, 24 hour, replicate 2 |
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| Relations |
| BioProject |
PRJNA120063 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17883_RAW.tar |
104.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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