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Series GSE178942 Query DataSets for GSE178942
Status Public on Jun 26, 2021
Title SARS-CoV-2 infected and uninfected VeroE6 and A549-ACE2: Bemcentinib and liposome treatments
Organisms Chlorocebus aethiops; Homo sapiens; Severe acute respiratory syndrome coronavirus 2
Experiment type Expression profiling by high throughput sequencing
Summary VeroE6 or A549-ACE2 cells were treated with inhibitors of phosphatidylserine receptors and infected with SARS-CoV-2.
 
Overall design VeroE6 or A549-ACE2 (lentiviral overexpression) were left untreated or treated with 1 micromolar bemcentinib for 1 hour, then challenged with a SARS-CoV-2 MOI of 0.01 (VeroE6) or 0.5 for either 18 hours (VeroE6) or 24 hours (A549-ACE2). Cells are lysed and mRNA isolated according to RNEasy miniprep protocol. Additional treatments are included, such as phsphatidylserine liposomes, phosphatidylcholine liposomes. 100000 reads were collected from each sample.
 
Contributor(s) Bohan D, Maury W
Citation(s) 34797899
Submission date Jun 25, 2021
Last update date Aug 16, 2022
Contact name Wendy Maury
Organization name University of Iowa
Department Microbiology and Immunology
Lab Maury Lab
Street address 51 Newton Rd, Bowen Science Building 3-615
City Iowa City
State/province Iowa
ZIP/Postal code 52242
Country USA
 
Platforms (4)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
GPL29320 Illumina NovaSeq 6000 (Homo sapiens; Severe acute respiratory syndrome coronavirus 2)
GPL29682 Illumina NovaSeq 6000 (Chlorocebus aethiops)
Samples (70)
GSM5401607 1-Vero_E6-no_infection_lane1_20200605000
GSM5401608 2-Vero_E6-no_infection_lane1_20200605000
GSM5401609 3-Vero_E6-no_infection_lane1_20200605000
Relations
BioProject PRJNA741531
SRA SRP325675

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE178942_Counts_genocode_v26_BergenBio_200626_summary.xlsx 12.9 Kb (ftp)(http) XLSX
GSE178942_RAW.tar 200.0 Kb (http)(custom) TAR (of TXT)
GSE178942_summary_table_A549_GencodeV26_200616.xlsx 17.0 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record
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