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| Status |
Public on Feb 04, 2022 |
| Title |
Total RNA sequencing of mouse LSK cells in E14.5 fetal liver |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The study is to obtain a transcriptome-wide map of prenatal murine stem and progenitor cells. VavCre+ mice were used for conditional knockout of Ddx41. Wild-type, Ddx41 heterozygous (Ddx41+/-) and Ddx41 knockout (Ddx41-/-) LSK cells from embryonic 14.5 day fetal liver were harvested (three samples from each group) for RNA. Gene expression analysis and alternative splicing analysis were performed. RNA-seq data were confirmed by RT-qPCR and regular PCR followed by gel quantification. A global change of gene expression and substantial differential splicing events were observed in KO samples compared to WT and het.
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| Overall design |
Total RNA profiling of E14.5 fetal liver LSK cells of WT (Ddx41+/+), het (Ddx41+/-) and KO (Ddx41-/-) mice.
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| |
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| Contributor(s) |
Ross SR, Ma J |
| Citation(s) |
35303436 |
| |
| Submission date |
Jun 27, 2021 |
| Last update date |
Apr 27, 2022 |
| Contact name |
Susan Ross |
| E-mail(s) |
srross@uic.edu
|
| Phone |
312-996-4945
|
| Organization name |
University of Illinois at Chicago
|
| Department |
Microbiology & Immunology
|
| Street address |
835 South Wolcott Ave
|
| City |
Chicago |
| ZIP/Postal code |
60612 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA741785 |
| SRA |
SRP325771 |
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