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Series GSE178979 Query DataSets for GSE178979
Status Public on Feb 04, 2022
Title Total RNA sequencing of mouse LSK cells in E14.5 fetal liver
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The study is to obtain a transcriptome-wide map of prenatal murine stem and progenitor cells. VavCre+ mice were used for conditional knockout of Ddx41. Wild-type, Ddx41 heterozygous (Ddx41+/-) and Ddx41 knockout (Ddx41-/-) LSK cells from embryonic 14.5 day fetal liver were harvested (three samples from each group) for RNA. Gene expression analysis and alternative splicing analysis were performed. RNA-seq data were confirmed by RT-qPCR and regular PCR followed by gel quantification. A global change of gene expression and substantial differential splicing events were observed in KO samples compared to WT and het.
Overall design Total RNA profiling of E14.5 fetal liver LSK cells of WT (Ddx41+/+), het (Ddx41+/-) and KO (Ddx41-/-) mice.
Contributor(s) Ross SR, Ma J
Citation(s) 35303436
Submission date Jun 27, 2021
Last update date Apr 27, 2022
Contact name Susan Ross
Phone 312-996-4945
Organization name University of Illinois at Chicago
Department Microbiology & Immunology
Street address 835 South Wolcott Ave
City Chicago
ZIP/Postal code 60612
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (9)
GSM5402595 LSK cells-WT1 [W_1]
GSM5402596 LSK cells-WT2 [W_L2]
GSM5402597 LSK cells-WT3 [W_L5]
BioProject PRJNA741785
SRA SRP325771

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Supplementary file Size Download File type/resource
GSE178979_RAW.tar 18.9 Mb (http)(custom) TAR (of XLSX)
GSE178979_Raw_gene_counts_matrix.xlsx 1.3 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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