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Series GSE178981 Query DataSets for GSE178981
Status Public on Nov 27, 2023
Title Runx1+ vascular smooth muscle cells are essential for hematopoietic stem and progenitor cell development in vivo
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Hematopoietic stem cells (HSCs) produce all essential cellular components of the blood. Stromal cell lines supporting HSCs follow a vascular smooth muscle cell (vSMC) differentiation pathway, suggesting that some hematopoiesis-supporting cells originate from vSMC precursors. These pericyte-like precursors were recently identified in the aorta-gonad-mesonephros (AGM) region; however, their role in in vivo hematopoietic development remains unknown. Here, we identify a subpopulation of NG2+Runx1+ perivascular cells that display a sclerotome-derived vSMC transcriptomic profile. We show that deleting Runx1 in NG2+ cells impairs in vivo hematopoietic development and causes transcriptional changes in pericytes/vSMCs, endothelial cells and hematopoietic cells in the murine AGM. Importantly, this deletion leads also to a signification reduction of HSC reconstitution potential in the bone marrow in vivo. This defect is developmental, as NG2+Runx1+ cells were not detected in the adult bone marrow, demonstrating the existence of a specialised pericyte population in the HSC-generating niche, unique to the embryo.
Overall design Single-cell transcriptome profiling of E11 AGM samples from NG2 +/+; Runx1 fl/fl (WT) (n=1) and NG2-Cre; Runx1 fl/fl (cKO) (n=1) mice
Contributor(s) Gonzalez ZN, Kilpatrick AM, Marques M, Sá da Bandeira D, Ventura T, Gomez-Salazar M, Bouilleau L, Marc Y, Barbosa A, Rossi F, Beltran M, van de Werken H, van IJcken W, Henderson NC, Forbes SJ, Crisan M
Citation(s) 38395882
Submission date Jun 27, 2021
Last update date Feb 26, 2024
Contact name Mihaela Crisan
Organization name The University of Edinburgh
Street address 5 Little France Drive
City Edinburgh
ZIP/Postal code EH16 4UU
Country United Kingdom
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM5402607 E11 AGM cells - NG2 +/+; Runx1 fl/fl (WT)
GSM5402608 E11 AGM cells - NG2-Cre; Runx1 fl/fl (cKO)
BioProject PRJNA741787
SRA SRP325765

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Supplementary file Size Download File type/resource
GSE178981_RAW.tar 493.6 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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