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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 27, 2023 |
| Title |
Runx1+ vascular smooth muscle cells are essential for hematopoietic stem and progenitor cell development in vivo |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hematopoietic stem cells (HSCs) produce all essential cellular components of the blood. Stromal cell lines supporting HSCs follow a vascular smooth muscle cell (vSMC) differentiation pathway, suggesting that some hematopoiesis-supporting cells originate from vSMC precursors. These pericyte-like precursors were recently identified in the aorta-gonad-mesonephros (AGM) region; however, their role in in vivo hematopoietic development remains unknown. Here, we identify a subpopulation of NG2+Runx1+ perivascular cells that display a sclerotome-derived vSMC transcriptomic profile. We show that deleting Runx1 in NG2+ cells impairs in vivo hematopoietic development and causes transcriptional changes in pericytes/vSMCs, endothelial cells and hematopoietic cells in the murine AGM. Importantly, this deletion leads also to a signification reduction of HSC reconstitution potential in the bone marrow in vivo. This defect is developmental, as NG2+Runx1+ cells were not detected in the adult bone marrow, demonstrating the existence of a specialised pericyte population in the HSC-generating niche, unique to the embryo.
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| Overall design |
Single-cell transcriptome profiling of E11 AGM samples from NG2 +/+; Runx1 fl/fl (WT) (n=1) and NG2-Cre; Runx1 fl/fl (cKO) (n=1) mice
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| Contributor(s) |
Gonzalez ZN, Kilpatrick AM, Marques M, Sá da Bandeira D, Ventura T, Gomez-Salazar M, Bouilleau L, Marc Y, Barbosa A, Rossi F, Beltran M, van de Werken H, van IJcken W, Henderson NC, Forbes SJ, Crisan M |
| Citation(s) |
38395882 |
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| Submission date |
Jun 27, 2021 |
| Last update date |
Feb 26, 2024 |
| Contact name |
Mihaela Crisan |
| E-mail(s) |
mihaela.crisan@ed.ac.uk
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| Organization name |
The University of Edinburgh
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| Street address |
5 Little France Drive
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| City |
Edinburgh |
| ZIP/Postal code |
EH16 4UU |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
| GSM5402607 |
E11 AGM cells - NG2 +/+; Runx1 fl/fl (WT) |
| GSM5402608 |
E11 AGM cells - NG2-Cre; Runx1 fl/fl (cKO) |
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| Relations |
| BioProject |
PRJNA741787 |
| SRA |
SRP325765 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE178981_RAW.tar |
493.6 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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