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Series GSE179291 Query DataSets for GSE179291
Status Public on Sep 04, 2021
Title bmp4 RNAi gene expression profiling
Organism Schmidtea mediterranea
Experiment type Expression profiling by high throughput sequencing
Summary Regeneration is a widespread phenomenon that often requires formation of a new tissue outgrowth at wounds called a blastema. Identifying the key signals that trigger blastema formation is therefore fundamental for understanding the mechanistic basis of regeneration. We uncovered a role for the wound epidermis in regeneration initiation in planarians. The gene equinox is expressed within hours of injury in the planarian wound epidermis and encodes a secreted protein conserved in many phyla. Following equinox inhibition, animals failed to form blastemas, reset positional information, and upregulate stem cell (neoblast) proliferation at wounds. Associated with these defects was an inability to maintain wound-induced gene expression. We propose that activation of equinox in the wound epidermis initiates planarian regeneration.
 
Overall design Polyadenylated RNA was isolated from post-pharyngeal fragments at 0, 3, 6, 16, 24, and 48 hours post-amputation (hpa) from planarians following RNAi of a control gene (C.elegans unc-22; 5 replicates) and bmp4 (5 replicates). Worms were fed four times over two weeks, and cut 7 days after 4th feeding.
 
Contributor(s) Cloutier J, Scimone L, Maybrun C, Reddien P
Citation(s) 35585061
Submission date Jul 01, 2021
Last update date May 27, 2022
Contact name Peter W Reddien
Organization name Whitehead Institute for Biomedical Research
Department MIT Biology
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platforms (1)
GPL15553 Illumina HiSeq 2000 (Schmidtea mediterranea)
Samples (60)
GSM5413721 post-pharyngeal fragments, u22_0h_1
GSM5413722 post-pharyngeal fragments, u22_0h_2
GSM5413723 post-pharyngeal fragments, u22_0h_3
This SubSeries is part of SuperSeries:
GSE179295 equinox is a planarian wound epidermis gene required for the initiation of regeneration
Relations
BioProject PRJNA743073
SRA SRP326526

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE179291_bmp_DEseq_Table.xlsx 11.5 Mb (ftp)(http) XLSX
GSE179291_bmp_reads_collapsed_v6_reorder.txt.gz 2.0 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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