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| Status |
Public on Feb 17, 2022 |
| Title |
Expression data from mouse lung epithelial cells during E. coli pneumonia in the presence or absence of LIF neutralization (24 h) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Leukemia inhibitory factor (LIF) is a cytoprotective cytokine that reduces lung injury during pneumonia. The purpose of this study was to determine the influence of LIF on pneumonia-induced gene changes in lung epithelium.
Mice received intratracheal instillations of vehicle (PBS) or Escherichia coli (10^6 CFU). Control IgG or anti-LIF specific IgG was included in the instillate. 24 hours after the challenge, lung epithelial cells (EpCAM+/CD45-) were sorted from enzymatic lung digests and used for RNA isolation and microarray analysis.
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| Overall design |
RNA from 3 separate groups of mice (3 mice per group) was analyzed: 1) 24h Vehicle/control IgG-treated; 2) 24h E. coli/control IgG-treated; and 3) 24h E. coli/anti-LIF-specific IgG.
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| Contributor(s) |
Na E, Traber K, Quinton L |
| Citation(s) |
35137631 |
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| Submission date |
Jul 04, 2021 |
| Last update date |
Jul 26, 2022 |
| Contact name |
Boston University Microarray and Sequencing Resource |
| E-mail(s) |
msrdata@bu.edu
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| Organization name |
Boston University
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| Department |
Microarray and Sequencing Resource
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| Street address |
72 East Concord Street, E631
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
| GPL21783 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: mogene10st_Mm_ENTREZG_17.0.0] |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE179764 |
Epithelial LIF signaling limits apoptosis and lung injury during bacterial pneumonia |
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| Relations |
| BioProject |
PRJNA743658 |
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