|
Status |
Public on Feb 17, 2022 |
Title |
Expression data from mouse lung epithelial cells during E. coli pneumonia in the presence or absence of LIF neutralization (6 h) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
Leukemia inhibitory factor (LIF) is a cytoprotective cytokine that reduces lung injury during pneumonia. The purpose of this study was to determine the influence of LIF on pneumonia-induced gene changes in lung epithelium. Mice received intratracheal instillations of vehicle (PBS) or Escherichia coli (10^6 CFU). Control IgG or anti-LIF specific IgG was included in the instillate. 6 hours after the challenge, lung epithelial cells (EpCAM+/CD45-) were sorted from enzymatic lung digests and used for RNA isolation and microarray analysis.
|
|
|
Overall design |
RNA from 3 separate groups of mice (3 mice per group) was analyzed: 1) 6h Vehicle/control IgG-treated; 2) 6h E. coli/control IgG-treated; and 3) 6h E. coli/anti-LIF-specific IgG.
|
|
|
Contributor(s) |
Na E, Traber K, Quinton L |
Citation(s) |
35137631 |
|
Submission date |
Jul 04, 2021 |
Last update date |
Jul 26, 2022 |
Contact name |
Boston University Microarray and Sequencing Resource |
E-mail(s) |
msrdata@bu.edu
|
Organization name |
Boston University
|
Department |
Microarray and Sequencing Resource
|
Street address |
72 East Concord Street, E631
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02118 |
Country |
USA |
|
|
Platforms (1) |
GPL21783 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: mogene10st_Mm_ENTREZG_17.0.0] |
|
Samples (9)
|
|
This SubSeries is part of SuperSeries: |
GSE179764 |
Epithelial LIF signaling limits apoptosis and lung injury during bacterial pneumonia |
|
Relations |
BioProject |
PRJNA743664 |