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Series GSE179474 Query DataSets for GSE179474
Status Public on Sep 30, 2021
Title Operation of the atypical canonical BMP signaling pathway during human odontogenesis
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary BMP (bone morphogenetic protein) signaling plays essential roles in the regulation of early tooth development. It is well acknowledged that with binding of extracellular BMP ligands to the type I and type II transmembrane serine/threonine kinase receptor complexes when triggering of the BMP canonical signaling pathway, receptor activated Smad1/5/8 in cytoplasm bind to Smad4, the central mediator of the canonical BMP signaling pathway, to form transfer complexes for entering the nucleus and regulating target gene expression. However, our recent studies reveal the functional operation of a novel BMP mediated signaling pathway named as the atypical BMP canonical signaling pathway in mouse developing tooth, which is Smad1/5/8 dependent but Smad4 independent. In the current study, we investigated whether this atypical BMP canonical signaling is conserved in human odontogenesis. We showed that pSmad1/5/8 is required for expression of MSX1, a well-defined BMP signaling target gene, in human dental mesenchyme, but the typical BMP canonical signaling is indeed not operating in the early human developing tooth, as assessed by the absence of pSMAD1/5/8-SMAD4 complexes in the dental mesenchyme and expression of MSX1 and translocation of pSMAD1/5/8 induced by BMP4 protein is SMAD4-independent in dental mesenchymal cells. Moreover, RNA-Seq data sets comparing the transcriptome profiles of human dental mesenchymal cells with and without SMAD4 knockdown by siRNA displays unchanged expression profiles of pSMAD1/5/8 downstream target genes, further affirming the functional operation of the atypical canonical BMP signaling pathway in a manner of SMAD1/5/8-dependent but SMAD4-independent in the dental mesenchyme during early odontogenesis.
Overall design Examination of mRNA level in with and without BMP4, DMSO, Dorsomorphin, and SMAD4 siRNA TREATED human dental mesenchymal cells.
Contributor(s) Hu X, Zhang Y, Hu X
Citation(s) 35211032
Submission date Jul 05, 2021
Last update date Mar 04, 2022
Contact name XIAOXIAO HU
Organization name Fujian Normal University
Street address Qishan Branch
City FuZhou
ZIP/Postal code 350108
Country China
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (15)
GSM5418805 hDMCs-BSA control siRNA rep1
GSM5418806 hDMCs-BSA control siRNA rep2
GSM5418807 hDMCs-BSA control siRNA rep3
BioProject PRJNA743914
SRA SRP327018

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE179474_DESeq2_result_file_BMP4_vs_BSA.txt.gz 826.7 Kb (ftp)(http) TXT
GSE179474_DESeq2_result_file_Dorsomorphin_vs_DMSO.txt.gz 863.6 Kb (ftp)(http) TXT
GSE179474_DESeq2_result_file_SMAD4_siRNA_vs_control_siRNA.txt.gz 788.4 Kb (ftp)(http) TXT
GSE179474_Normalized_counts_file_BMP4_DMSO.txt.gz 360.2 Kb (ftp)(http) TXT
GSE179474_Normalized_counts_file_BMP4_Dorsomorphin.txt.gz 353.7 Kb (ftp)(http) TXT
GSE179474_Normalized_counts_file_BMP4_SMAD4_siRNA.txt.gz 330.5 Kb (ftp)(http) TXT
GSE179474_Normalized_counts_file_BMP4_control_siRNA.txt.gz 355.0 Kb (ftp)(http) TXT
GSE179474_Normalized_counts_file_BSA_control_siRNA.txt.gz 348.6 Kb (ftp)(http) TXT
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