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| Status |
Public on Sep 30, 2021 |
| Title |
Operation of the atypical canonical BMP signaling pathway during human odontogenesis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
BMP (bone morphogenetic protein) signaling plays essential roles in the regulation of early tooth development. It is well acknowledged that with binding of extracellular BMP ligands to the type I and type II transmembrane serine/threonine kinase receptor complexes when triggering of the BMP canonical signaling pathway, receptor activated Smad1/5/8 in cytoplasm bind to Smad4, the central mediator of the canonical BMP signaling pathway, to form transfer complexes for entering the nucleus and regulating target gene expression. However, our recent studies reveal the functional operation of a novel BMP mediated signaling pathway named as the atypical BMP canonical signaling pathway in mouse developing tooth, which is Smad1/5/8 dependent but Smad4 independent. In the current study, we investigated whether this atypical BMP canonical signaling is conserved in human odontogenesis. We showed that pSmad1/5/8 is required for expression of MSX1, a well-defined BMP signaling target gene, in human dental mesenchyme, but the typical BMP canonical signaling is indeed not operating in the early human developing tooth, as assessed by the absence of pSMAD1/5/8-SMAD4 complexes in the dental mesenchyme and expression of MSX1 and translocation of pSMAD1/5/8 induced by BMP4 protein is SMAD4-independent in dental mesenchymal cells. Moreover, RNA-Seq data sets comparing the transcriptome profiles of human dental mesenchymal cells with and without SMAD4 knockdown by siRNA displays unchanged expression profiles of pSMAD1/5/8 downstream target genes, further affirming the functional operation of the atypical canonical BMP signaling pathway in a manner of SMAD1/5/8-dependent but SMAD4-independent in the dental mesenchyme during early odontogenesis.
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| Overall design |
Examination of mRNA level in with and without BMP4, DMSO, Dorsomorphin, and SMAD4 siRNA TREATED human dental mesenchymal cells.
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| Contributor(s) |
Hu X, Zhang Y, Hu X |
| Citation(s) |
35211032 |
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| Submission date |
Jul 05, 2021 |
| Last update date |
Mar 04, 2022 |
| Contact name |
XIAOXIAO HU |
| E-mail(s) |
huxiao0221@msn.cn
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| Organization name |
Fujian Normal University
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| Street address |
Qishan Branch
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| City |
FuZhou |
| ZIP/Postal code |
350108 |
| Country |
China |
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| Platforms (1) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA743914 |
| SRA |
SRP327018 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE179474_DESeq2_result_file_BMP4_vs_BSA.txt.gz |
826.7 Kb |
(ftp)(http) |
TXT |
| GSE179474_DESeq2_result_file_Dorsomorphin_vs_DMSO.txt.gz |
863.6 Kb |
(ftp)(http) |
TXT |
| GSE179474_DESeq2_result_file_SMAD4_siRNA_vs_control_siRNA.txt.gz |
788.4 Kb |
(ftp)(http) |
TXT |
| GSE179474_Normalized_counts_file_BMP4_DMSO.txt.gz |
360.2 Kb |
(ftp)(http) |
TXT |
| GSE179474_Normalized_counts_file_BMP4_Dorsomorphin.txt.gz |
353.7 Kb |
(ftp)(http) |
TXT |
| GSE179474_Normalized_counts_file_BMP4_SMAD4_siRNA.txt.gz |
330.5 Kb |
(ftp)(http) |
TXT |
| GSE179474_Normalized_counts_file_BMP4_control_siRNA.txt.gz |
355.0 Kb |
(ftp)(http) |
TXT |
| GSE179474_Normalized_counts_file_BSA_control_siRNA.txt.gz |
348.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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