|
| Status |
Public on Feb 22, 2023 |
| Title |
CRISPRi screen for a-amylase yield improvements in Bacillus subtilis |
| Organism |
Bacillus subtilis subsp. subtilis str. 168 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Our study showed that optimizing ncRNA expression can increase or lower the yield of alpha-amylase enzyme production in Bacillus subtilis while revealing a range of potentially novel ncRNAs.
|
| |
|
| Overall design |
RNA-seq data for bacterial amylase production in two strains (codon optimized v. control) with two fed-batch replicates on two days
|
| |
|
| Contributor(s) |
Geissler AS, Ohrt Fehler A, Dahl Poulsen L, González-Tortuero E, Beuchert Kallehauge T, Alkan F, Anthon C, Seemann SE, Dolberg Rasmussen M, Breüner A, Hjort C, Vinther J, Gorodkin J |
| Citation(s) |
36564025 |
| |
| Submission date |
Jul 06, 2021 |
| Last update date |
Feb 22, 2023 |
| Contact name |
Christian Anthon |
| E-mail(s) |
anthon@rth.dk
|
| Phone |
+4521510929
|
| Organization name |
University of Copenhagen
|
| Department |
Veterinary and Animal Sciences
|
| Lab |
RTH
|
| Street address |
Thorvaldsens Vej 57
|
| City |
Frederiksberg C |
| ZIP/Postal code |
1871 |
| Country |
Denmark |
| |
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| Platforms (1) |
| GPL21796 |
Illumina NextSeq 500 (Bacillus subtilis subsp. subtilis str. 168) |
|
| Samples (8)
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|
| Relations |
| BioProject |
PRJNA744212 |
| SRA |
SRP327186 |
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