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Series GSE179622 Query DataSets for GSE179622
Status Public on Jul 31, 2022
Title Deficiency of IFT20 in CD4+ T cell alleviates protease-induced neutrophilic asthma-like inflammation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Many neutrophilic asthma patients do not respond to current medications, highlighting the need for novel therapeutic targets. Here, we investigated the role of intraflagellar transport (IFT) complex protein IFT20 in neutrophilic asthma. Mice lacking CD4+ T cell-specific IFT20 displayed reduced protease-induced neutrophilic asthma inflammation. Thus, IFT20 may represent a promising therapeutic target for treatment of patients with neutrophilic asthma.
 
Overall design We analyzed infiltrated immune cells in lung by single cell RNA sequencing (scRNAseq) with 10X genomics. C57BL/6 background Ift20f/f or Ift20f/f CD4 cre mice were administered 25-μg of papain intranasally for three consecutive days to sensitize them to the allergen. On day 14 after first administration of papain, mice were challenged with the same concentration of papain administered via intranasal injection. Immune cells were harvested from lung tissue on day 15.
 
Contributor(s) Jeong J, Kim CW, Kim HC, Choi YJ, Kim J, Lee HK
Citation(s) 37029318
Submission date Jul 07, 2021
Last update date Aug 10, 2023
Contact name Jiung Jeong
E-mail(s) skjung1371@kaist.ac.kr
Organization name KAIST
Lab E7 5101
Street address 291, Daehak-ro, Yuseong-gu
City Daejeon
ZIP/Postal code 34141
Country South Korea
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (4)
GSM5425188 DPBS-WT
GSM5425189 DPBS-KO
GSM5425190 Papain-WT
Relations
BioProject PRJNA744388
SRA SRP327310

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE179622_RAW.tar 243.2 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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