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Series GSE179623 Query DataSets for GSE179623
Status Public on Jul 31, 2022
Title IFT20 is important for effector function of CD4+ T cell
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Recent studies have suggested that formation of supramolecular activation cluster (SMAC), a large ‘immunological synapse’ composed of various signaling complexes, enhances CD4+ T cell activation. Critically, although the composition of SMAC is known, the proteins involved in SMAC formation have not been fully investigated. We identified that intraflagellar transport 20 (IFT20) play central roles in the effector functino of CD4+ T cells.
 
Overall design We analyzed in vitro activated CD4 T cell by single cell RNA sequencing (scRNAseq) with 10X genomics. CD4+ T cells for T cell activation were extracted from spleens of Ift20f/f or Ift20f/f CD4cre mice. Following single cell isolation, the MagnisortTM Mouse CD4 naïve T cell Enrichment Kit (Invitrogen) was used to obtain purified naïve T cells. Naïve CD4 + T cells were activated with 1-μg/mL anti-CD3 (Invitrogen) and 1-μg/mL anti-CD28 antibodies (BioLegend) for 24 hours.
 
Contributor(s) Jeong J, Kim CW, Kim HC, Choi YJ, Kim J, Lee HK
Citation(s) 37029318
Submission date Jul 07, 2021
Last update date Aug 10, 2023
Contact name Jiung Jeong
E-mail(s) skjung1371@kaist.ac.kr
Organization name KAIST
Lab E7 5101
Street address 291, Daehak-ro, Yuseong-gu
City Daejeon
ZIP/Postal code 34141
Country South Korea
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (3)
GSM5425192 0hr-WT-5pGEX
GSM5425193 24hr-WT-5pGEX
GSM5425194 24hr-KO-5pGEX
Relations
BioProject PRJNA744394
SRA SRP327307

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE179623_RAW.tar 262.3 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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