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| Status |
Public on Dec 23, 2021 |
| Title |
Identification of H3K27ac-marked enhancers in bovine satellite cells during differentiation |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study we isolated satellite cells from skeletal muscle from two Angus-crossbred steers, initially cultured them in growth medium for proliferation, and then in differentiation medium to induce them to differentiate and fuse into myotubes. We used ChIP-seq to identify enhancers marked with H3K27ac, i.e., active enhancers, from these cells immediately before and two days after induction of differentiation. Nearly 20,000 and 50,000 H3K27ac-marked enhancers were consistently identified from two biological replicates of before- and during-differentiation bovine satellite cells, respectively.
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| Overall design |
ChIP-seq was used to identify H3K27ac-marked genomic regions from bovine satellite cells immediately and two days after induction of differentiation. Two biological replicates were used. Four H3K27ac_ChIP-seq and two Input-seq libraries were sequenced.
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| |
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| Contributor(s) |
Jiang H, Lyu P, Settlage R |
| Citation(s) |
34915843 |
| Submission date |
Jul 09, 2021 |
| Last update date |
Dec 24, 2021 |
| Contact name |
Honglin Jiang |
| E-mail(s) |
hojiang@vt.edu
|
| Phone |
540-231-1859
|
| Organization name |
Virginia Polytechnic Institute and State University
|
| Department |
Animal and Poultry Sciences
|
| Street address |
3130 Litton Reaves Hall
|
| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24061 |
| Country |
USA |
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| Platforms (1) |
| GPL19172 |
Illumina HiSeq 2500 (Bos taurus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA745179 |
| SRA |
SRP327658 |
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