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| Status |
Public on Feb 17, 2022 |
| Title |
Single-cell RNA-seq of lung cells following bacterial pneumonia with or without LIF blockade |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
C57BL/6 mice were intratracheally challenged for 24 hours with saline or E. coli (1 x 10^6 CFU) with or without LIF neutralization. Left lobes were collected and digested into single-cell suspensions for FACS. Equal ratios of CD45+ (leukocytes), CD45-EpCAM+ (epithelium), and CD45-EpCAM- (other) cells were pooled from a mouse undergoing each condition.
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| Overall design |
Sorted whole lung C57BL/6 mouse cells 24 hours after IgG saline, IgG E. coli, or anti-LIF E. coli
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| Contributor(s) |
Xaralabos V, Elim N, Lee Q |
| Citation(s) |
35137631 |
| |
| Submission date |
Jul 10, 2021 |
| Last update date |
Feb 27, 2024 |
| Contact name |
Single Cell Sequencing Core |
| E-mail(s) |
scsdata@bu.edu
|
| Organization name |
BU Single Cell Sequencing Core
|
| Street address |
72 E. Concord St
|
| City |
Boston |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA745341 |
| SRA |
SRP327800 |
