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| Status |
Public on Jul 15, 2022 |
| Title |
single-cell RNA-seq of murine and human lungs by TAS-Seq |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Single-cell RNA-sequencing (scRNA-seq) is valuable for analyzing cellular heterogeneity. Cell composition accuracy is critical for analyzing cell-cell interaction networks from scRNA-seq data because cell abundancy might affect to the network. We developed terminator-assisted solid-phase cDNA amplification and sequencing (TAS-Seq), a scRNA-seq method that relied on terminator, terminal transferase, and nanowell/beads-based scRNA-seq platform, that could acquire scRNA-seq data with higher correlation with flow-cytometric data, gene-detection sensitivity, and robustness than widely-used methods.
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| Overall design |
To clarify performance of TAS-Seq, we analyzed 8-week-old C57BL/6J female mouse lung and lungs from lung cancer patients with pulmonary fibrosis who underwent curative surgical resection from August 2015 to December 2019 at Nara Medical University Hospital, who was diagnosed as rheumatoid arthritic-associated interstitial lung disease by TAS-Seq.
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| Contributor(s) |
Shichino S, Hashimoto S, Kitabatake M, Nakajima T, Ouji-Sageshima N, Hontsu S, Sawabata N, Kawaguchi T, Ueha S, Matsushima K |
| Citation(s) |
35760847 |
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| Submission date |
Jul 14, 2021 |
| Last update date |
Jul 17, 2022 |
| Contact name |
Shigeyuki Shichino |
| E-mail(s) |
s_shichino@rs.tus.ac.jp
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| Organization name |
Tokyo University of Science
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| Department |
Department of Molecular regulation of Inflammatory and Immune Diseases
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| Street address |
2641, Yamasaki, Noda-shi
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| City |
Chiba |
| ZIP/Postal code |
278-0022 |
| Country |
Japan |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA746669 |
| SRA |
SRP328400 |
