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| Status |
Public on Sep 12, 2009 |
| Title |
Fibrinogen-reactive T cells transfer disease to naïve mice |
| Platform organisms |
Homo sapiens; Mus musculus |
| Sample organism |
Mus musculus |
| Experiment type |
Protein profiling by protein array
|
| Summary |
Splenocytes harvested from mice with FIA were cultured in vitro and stimulated with 0.01 mg/ml fibrinogen for 3 days. Enriched T cells were transferred into 6-week-old naïve SJL mice, which developed visible signs of arthritis within 2 weeks. Synovial array profiling of plasma from the arthritic recipient mice demonstrated autoreactive B cell responses against peptides representing native fibrinogen, and further spreading of the responses to target collagen type V, cartilage gp39, and citrullinated vimentin.
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| Overall design |
Custom-spotted protein slides were with plasma samples from individual mice. Four slides were probed with plasma derived from naïve mice and five slides were probed with plasma derived from mice injected with FIA T cells.
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| Contributor(s) |
Ho PP, Lee LY, Zhao X, Tomooka BH, Paniagua RT, Sharpe O, BenBarak MJ, Hueber W, Chandra PE, Steinman L, Robinson WH |
| Citation(s) |
19949094 |
| Submission date |
Sep 09, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
William Robinson |
| E-mail(s) |
wrobins@stanford.edu
|
| Phone |
6508491207
|
| Fax |
6508491208
|
| Organization name |
Stanford University
|
| Street address |
269 Campus Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL9165 |
Synovial protein array 0.5K v2.0 |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE18073 |
Mice with fibrinogen-induced arthritis (FIA) |
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| Relations |
| BioProject |
PRJNA123465 |
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