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| Status |
Public on Sep 13, 2021 |
| Title |
RNA-seq analysis of MEF cells with stably overexpression of IDH2 mutation and MEF cells treated with 2-HG |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Isocitrate dehydrogenase 2 (IDH2) mutations and its key effector 2-hydroxyglutarate (2-HG) have been reported to promote oncogenesis in various human cancers. To elucidate molecular mechanism(s) associated with IDH2 mutations, we established mouse embryonic fibroblasts (MEF) cells stably expressing cancer-associated IDH2R172S mutation and treated MEF cells with or without 2-HG. The expression of genes in these cells were analyzed by RNA-seq.
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| Overall design |
Expression profiles of MEF cells stably expressing mutant IDH2 or mock vector, and MEF cells treated with 300 μM of 2-HG or DMSO for 24 h
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| Web link |
https://doi.org/10.1371/journal.pone.0257090
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| |
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| Contributor(s) |
Liu X, Ikenoue T, Furukawa Y, Yamaguchi K |
| Citation(s) |
34516556 |
| |
| Submission date |
Jul 19, 2021 |
| Last update date |
Dec 13, 2021 |
| Contact name |
XUN LIU |
| Organization name |
The University of Tokyo
|
| Street address |
Shirokanedai 4-6-1
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| City |
Minato-ku, Tokyo |
| ZIP/Postal code |
108-8639 |
| Country |
Japan |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA748020 |
| SRA |
SRP328943 |
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