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Series GSE180610 Query DataSets for GSE180610
Status Public on Sep 01, 2023
Title Differential gene expression in MEF SEC24D knockout cells with additional SEC24s knockdown
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We transfected MEF SEC24D KO cells with SEC24B siRNA for a double KD or combined SEC24s siRNA (SEC24a, 24B and 24C) for a quadruple KD. Cells were transfected with siRNA or Scramble siRNA (control) for 2 days then total RNA for each knockdown sample was extracted and purified for RNA-seq to evaluate the change in transcriptome.
 
Overall design Examination of gene expression in Sec24D knockdown
 
Contributor(s) Kim J, Lu C
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jul 21, 2021
Last update date Sep 01, 2023
Contact name Jinoh Kim
E-mail(s) jinohk@iastate.edu
Phone 5152943401
Organization name Iowa State University
Department Biomedical Sciences
Lab 2086 Vet Med
Street address 515 Morrill Rd
City Ames
State/province IA
ZIP/Postal code 50011
Country USA
 
Platforms (1)
GPL15103 Illumina HiSeq 1000 (Mus musculus)
Samples (19)
GSM5465995 MEF Sec24D KO treated with siRNA knockdown for Sec24A, Sec24B, & Sec24C [ABCD.1]
GSM5465996 MEF Sec24D KO treated with siRNA knockdown for Sec24A, Sec24B, & Sec24C [ABCD.2]
GSM5465997 MEF Sec24D KO treated with siRNA knockdown for Sec24A, Sec24B, & Sec24C [ABCD.3]
Relations
BioProject PRJNA748815
SRA SRP329348

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE180610_processeddata.xlsx 5.6 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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