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| Status |
Public on Jul 23, 2021 |
| Title |
Genome-wide gene expression of NB-1 after treatment of 5-aza-dC, TBT, and combination |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A neuroblastoma cell line, NB-1, was treated with mock, a DNA demethylating agent (5-aza-2'-deoxycytidine: 5-aza-dC), a synthetic retinoic acid (tamibarotene: TBT), and the combination of 5-aza-dC and TBT. A genome-wide gene expression analysis was performed using SurePrint G3 Human Gene Expression 8 x 60K v2 Microarray.
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| Overall design |
Cy3-labeled cRNA was synthesized from 200 ng of total RNA using a Low Input Quick Amp Labeling Kit, and 600 ng of labeled cRNA was fragmented and hybridized to SurePrint G3 Human GE 8x60K Microarray Ver2.0 Microarray.
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| Contributor(s) |
Hattori N, Ushijima T |
| Citation(s) |
34635821 |
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| Submission date |
Jul 22, 2021 |
| Last update date |
Oct 22, 2021 |
| Contact name |
Naoko Hattori |
| E-mail(s) |
nhattori@ncc.go.jp
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| Phone |
+81-3-3542-2511
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| Organization name |
National Cancer Center
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| Department |
Research Institute
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| Lab |
Division of Epigenomics
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| Street address |
5-1-1 Tsukiji, Chuo-ku
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| City |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA749022 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE180645_RAW.tar |
12.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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