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Status |
Public on Aug 05, 2024 |
Title |
Expression data changes induced by 3D conditions in T-ALL cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
3D constrained conditions promoted H3K4 methylation in ALL cells that altered the global chromatin configuration and transcriptional changes related to cell cycle and DNA replication.
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Overall design |
Jurkat cells (T-ALL cell line) were used for RNA extraction and hybridization on Affymetrix microarrays. Jurkat cells were cultured in suspension or 3D conditions (a type I collagen matrix of 1,7 mg/mL) for 3 hours. Cellsd were lised and mRNA was extracted to compare expressions profiles. We used three replicates of each sample.
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Contributor(s) |
González-Novo R, González-Murillo Á, Madrazo E, Acitores D, García de Lacoba M, Ramírez M, Redondo-Muñoz J |
Citation missing |
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Submission date |
Aug 03, 2021 |
Last update date |
Aug 05, 2024 |
Contact name |
Javier Redondo-Muñoz |
E-mail(s) |
javier.redondo@cib.csic.es
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Organization name |
CIB-CSIC
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Department |
Molecular Biomedicine
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Street address |
Ramiro de Maeztu,9
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City |
Madrid |
State/province |
Madrid |
ZIP/Postal code |
28040 |
Country |
Spain |
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Platforms (1) |
GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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Samples (6)
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Relations |
BioProject |
PRJNA751855 |