GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE18141 Query DataSets for GSE18141
Status Public on Oct 02, 2009
Title Gene expression profile of csr-1(tm892) animals compared to wild type
Organism Caenorhabditis elegans
Experiment type Expression profiling by genome tiling array
Summary RNAi-related pathways regulate diverse processes, from developmental timing to transposon silencing. Here, we show that in C. elegans the Argonaute CSR-1, the RNA-dependent RNA polymerase EGO-1, the Dicer-related helicase DRH-3, and the Tudor-domain protein EKL-1 localize to chromosomes and are required for proper chromosome segregation. In the absence of these factors chromosomes fail to align at the metaphase plate and kinetochores do not orient to opposing spindle poles. Surprisingly, the CSR-1 interacting small RNAs (22G-RNAs) are antisense to thousands of germline-expressed protein-coding genes. Nematodes assemble holocentric chromosomes in which continuous kinetochores must span the expressed domains of the genome. We show that CSR-1 interacts with chromatin at target loci, but does not down-regulate target mRNA or protein levels. Instead, our findings support a model in which CSR-1 complexes target protein-coding domains to promote their proper organization within the holocentric chromosomes of C. elegans.
Overall design Total RNA was extracted from C. elegnas csr-1(tm892) and wild type animals
Contributor(s) Claycomb JM, Mello CC
Citation(s) 19804758
Submission date Sep 16, 2009
Last update date Apr 30, 2013
Contact name Julie M. Claycomb
Organization name University of Massachusetts Medical School
Department Program in Molecular Medicine
Lab Craig Mello
Street address 373 Plantation Street Biotech2 Suite 219
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
Platforms (1)
GPL5634 [Ce25b_MR] Affymetrix C. elegans Tiling 1.0R Array
Samples (2)
GSM453630 CSR-1 (tm892)
GSM453631 Wild-type
This SubSeries is part of SuperSeries:
GSE18167 Gene expression in csr-1 mutants and sequencing of sRNAs from CSR-1 IP complexes and csr-1 and ego-1 mutants
BioProject PRJNA123481

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18141_RAW.tar 185.2 Mb (http)(custom) TAR (of BAR, CEL)
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap