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| Status |
Public on Oct 02, 2009 |
| Title |
Gene expression profile of csr-1(tm892) animals compared to wild type |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by genome tiling array
|
| Summary |
RNAi-related pathways regulate diverse processes, from developmental timing to transposon silencing. Here, we show that in C. elegans the Argonaute CSR-1, the RNA-dependent RNA polymerase EGO-1, the Dicer-related helicase DRH-3, and the Tudor-domain protein EKL-1 localize to chromosomes and are required for proper chromosome segregation. In the absence of these factors chromosomes fail to align at the metaphase plate and kinetochores do not orient to opposing spindle poles. Surprisingly, the CSR-1 interacting small RNAs (22G-RNAs) are antisense to thousands of germline-expressed protein-coding genes. Nematodes assemble holocentric chromosomes in which continuous kinetochores must span the expressed domains of the genome. We show that CSR-1 interacts with chromatin at target loci, but does not down-regulate target mRNA or protein levels. Instead, our findings support a model in which CSR-1 complexes target protein-coding domains to promote their proper organization within the holocentric chromosomes of C. elegans.
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| Overall design |
Total RNA was extracted from C. elegnas csr-1(tm892) and wild type animals
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| Contributor(s) |
Claycomb JM, Mello CC |
| Citation(s) |
19804758 |
| |
| Submission date |
Sep 16, 2009 |
| Last update date |
Apr 30, 2013 |
| Contact name |
Julie M. Claycomb |
| Organization name |
University of Massachusetts Medical School
|
| Department |
Program in Molecular Medicine
|
| Lab |
Craig Mello
|
| Street address |
373 Plantation Street Biotech2 Suite 219
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
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| Platforms (1) |
| GPL5634 |
[Ce25b_MR] Affymetrix C. elegans Tiling 1.0R Array |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE18167 |
Gene expression in csr-1 mutants and sequencing of sRNAs from CSR-1 IP complexes and csr-1 and ego-1 mutants |
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| Relations |
| BioProject |
PRJNA123481 |
