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Status |
Public on Aug 17, 2021 |
Title |
Bulk RNA sequencing of human intestinal and airway organoids |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Bulk RNA sequencing was performed on intestinal and airway organoids. Also airway organoid derived air-liquid interface cultures were sequenced
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Overall design |
RNA was extracted from intestinal organoids that were either grown in expansion conditions (EXP) or differentiation conditions (DIF). Airway organoids were grown in expansion medium (EM) or differentiated in differentiation medium (DM). Air-liquid interface cultures of trachea, bronchus and bronchiole were derived from airway organoids of that airway section.
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Contributor(s) |
Beumer J, Geurts MH, Lamers MM, Puschhof J, Zhang J, van der Vaart J, Mykytyn AZ, Breugem TI, Riesebosch S, Schipper D, van den Doel PB, de Lau W, Pleguezuelos-Manzano C, Busslinger G, Haagmans BL, Clevers H |
Citation(s) |
- Beumer J, Geurts MH, Lamers MM, Puschhof J et al. A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses. Nat Commun 2021 Sep 17;12(1):5498. PMID: 34535662
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Submission date |
Aug 05, 2021 |
Last update date |
Oct 13, 2021 |
Contact name |
Jelte van der Vaart |
E-mail(s) |
j.vaart@hubrecht.eu
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Organization name |
Hubrecht Institute
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Lab |
dr. Hans Clevers Lab
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Street address |
Uppsalalaan 8
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City |
Utrecht |
ZIP/Postal code |
3584CT |
Country |
Netherlands |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (14)
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Relations |
BioProject |
PRJNA752330 |
SRA |
SRP331223 |
Supplementary file |
Size |
Download |
File type/resource |
GSE181514_KOpaper_ndata.csv.gz |
1.0 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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