|
| Status |
Public on Jan 20, 2023 |
| Title |
mNET-seq on COMPASS-degron cells |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
We measured the the dwell time of existed paused RNAPII at the promoter by mammalian native elongating transcript sequencing (mNET-seq). We found a median RNAPII half-life of 5.3 min at protein-coding genes in DMSO pre-treated control mESCs, whereas the RNAPII half-life at these genes significantly slowdown to 8.96 min when pre-treated with Auxin in the DPY30-mAID degron cell. The data suggested that loss H3K4me3 directly slows down the release of paused RNAPII throughout genes.
|
| |
|
| Overall design |
To directly investigate the temporal stability and half-life of promoter RNAPII, we used Triptolide, a potent inhibitor of transcription initiation, coupled with mammalian native elongating transcript sequencing (mNET-seq) to measure the dwell time of existed paused RNAPII at the promoter. Unlike previous measurements, the single-nucleotide resolution of the mNET-seq data allowed us to specifically direct measure the RNAPII engaged RNA signal at protomer-proximal region.
|
| |
|
| Contributor(s) |
Wang H |
| Citation(s) |
36859550 |
| |
| Submission date |
Aug 09, 2021 |
| Last update date |
Mar 12, 2023 |
| Contact name |
Hua Wang |
| E-mail(s) |
moodswh@gmail.com
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Street address |
430 East 67th Street
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (20)
|
|
| This SubSeries is part of SuperSeries: |
|
| Relations |
| BioProject |
PRJNA753050 |
| SRA |
SRP331638 |
Less...
More...