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Series GSE181765 Query DataSets for GSE181765
Status Public on Jul 31, 2022
Title Oxidative Phosphorylation Regulates Interleukin-10 Production in Regulatory B Cells via the Extracellular Signal-related Kinase Pathway
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Regulatory B cells (Bregs) are immune cells that constrain the immune response and restrict inflammation via their expression of interleukin (IL)-10. However, the molecular mechanisms underlying Breg differentiation and IL-10 secretion remain unclear. Previous data suggest that cellular metabolism determines both the fate and function of these cells. Here, we suggest an essential role for mitochondrial oxidative phosphorylation (OXPHOS) in the regulation of IL-10 in these Bregs. We found that IL-10+ B cells from IL-10-green fluorescent protein-expressing mice had higher oxygen consumption rate than IL-10- B cells. In addition, inhibition of OXPHOS decreased the expression of IL-10 in B cells. Further, suppression of OXPHOS diminished the expression of surface markers for Bregs and impaired their therapeutic effects in dextran sulfate sodium-induced colitis. Mechanistically, mitochondrial OXPHOS was found to regulate the transcription factor HIF-1α through the extracellular signal-related kinase pathway. Taken together, this study reveals a strong correlation between mitochondrial OXPHOS and Breg phenotype/function, indicating OXPHOS as a therapeutic target in autoimmune diseases driven by Breg dysfunction.
 
Overall design After splenic B cells from IL-10-eGFP Tiger mice were stimulated with 3 µg/ml LPS for 48 h, IL-10+ B cells and IL-10- B cells were sorted.And mRNA profiles of them were examined.
 
Contributor(s) Zhu Y, Zhang X, Xie S, Peng Y
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Submission date Aug 10, 2021
Last update date Jul 31, 2022
Contact name Yinhong Zhu
E-mail(s) zhuyh35@mail2.sysu.edu.cn
Phone 15622177757
Organization name
Street address zhongshan Second Road
City guangzhou
State/province guangdong
ZIP/Postal code 510000
Country China
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM5511032 IL-10+ B cells rep1
GSM5511033 IL-10- B cells rep1
GSM5511034 IL-10+ B cells rep2
Relations
BioProject PRJNA753317
SRA SRP331844

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE181765_gene_expression_FPKM.txt.gz 1.0 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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