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Series GSE182058 Query DataSets for GSE182058
Status Public on Dec 04, 2023
Title The effect of PHZ-induced hemolytic anemia on HSCs [bisulfite-seq]
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Hematopoietic stem cells (HSCs) are capable of regenerating the blood system, but the instructive cues that direct HSCs to regenerate particular lineages lost to the injury remain elusive. Here, we show that iron is increasingly taken up by HSCs during anemia and induces erythroid gene expression and regeneration in a Tet2-dependent manner. Lineage tracing of HSCs revealed that HSCs respond to hemolytic anemia by increasing erythroid output. The number of HSCs in the spleen, but not bone marrow, increased upon anemia and these HSCs exhibited enhanced proliferation, erythroid differentiation, iron uptake, and TET2 protein expression. Increased iron in HSCs promoted DNA demethylation and expression of erythroid genes. Suppressing iron uptake or TET2 expression impaired erythroid genes expression and erythroid differentiation of HSCs; iron supplementation, however, augmented these processes. These results establish that the physiological level of iron taken up by HSCs has an instructive role in promoting erythroid-biased differentiation of HSCs.
Overall design Bisulfite-seq in control bone marrow HSCs and PHZ-treated bone marrow and splenic HSCs. The WT and PHZ-treated bone marrow HSC samples have 3 replicates each, the WT splenic HSC samples have 2 replicates, and the PHZ-treated splenic HSC samples have 3 replicates.
Contributor(s) Tseng Y, Murdaugh RL, Nakada D
Citation(s) 38225226
Submission date Aug 13, 2021
Last update date Jan 29, 2024
Contact name Rebecca Murdaugh
Organization name Baylor College of Medicine
Street address One Baylor Plaza
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (11)
GSM5517032 WT_BM_HSC_1 [bisulfite-seq]
GSM5517033 WT_BM_HSC_2 [bisulfite-seq]
GSM5517034 WT_BM_HSC_3 [bisulfite-seq]
This SubSeries is part of SuperSeries:
GSE182059 The effect of PHZ-induced hemolytic anemia on HSCs
BioProject PRJNA754388
SRA SRP332449

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE182058_RAW.tar 30.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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