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| Status |
Public on Oct 01, 2009 |
| Title |
Expression comparison between C. elegans drh-3(ne4253) and wild-type |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by genome tiling array
|
| Summary |
Endogenous small RNAs (endo-siRNAs) interact with Argonaute (AGO) proteins to mediate sequence-specific regulation of diverse biological processes. Here, we combine deep-sequencing and genetic approaches to explore the biogenesis and function of endo-siRNAs in C. elegans. We describe conditional alleles of the dicer-related helicase, drh-3, that abrogate both RNA interference and the biogenesis of endo-siRNAs, called 22G-RNAs. DRH-3 is a core component of RNA-dependent RNA polymerase (RdRP) complexes essential for several distinct 22G-RNA systems. We show that in the germ-line, one system is dependent on worm-specific AGOs, including WAGO-1, which localizes to germ-line nuage structures called P-granules. WAGO-1 silences certain genes, transposons, pseudogenes and cryptic loci. Finally, we demonstrate that components of the nonsense-mediated decay pathway function in at least one WAGO-mediated surveillance pathway. These findings broaden our understanding of the biogenesis and diversity of 22G-RNAs and suggest novel regulatory functions for small RNAs.
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| Overall design |
Total RNA was extracted from C. elegans drh-3(ne4253) and wild type animals
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| Contributor(s) |
Gu W, Mello CC |
| Citation(s) |
19800275 |
| |
| Submission date |
Sep 23, 2009 |
| Last update date |
Apr 30, 2013 |
| Contact name |
Craig Mello |
| Organization name |
University of Massachusetts Medical School
|
| Department |
Program in Molecular Medicine
|
| Lab |
Craig Mello
|
| Street address |
368 Plantatoin Street, Suite AS5-2047
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL5634 |
[Ce25b_MR] Affymetrix C. elegans Tiling 1.0R Array |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA123501 |
