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| Status |
Public on Sep 01, 2021 |
| Title |
Epithelial XBP1 coordinates TP53-driven DNA damage responses and suppression of intestinal carcinogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We assessed the impact of intestinal epithelial XBP1 in coordinating epithelial DNA damage responses. As our data revealed that low XBP1 activity in the context of chronic DNA damage is associated with both reduced p53 pathway activity and formation of tumors with metastaic potential in-vivo, we performed RNA sequencing of intestinal organoids (H2b/Xbp1fl/fl, H2bΔIEC, H2b/Xbp1ΔIEC, H2b/p53∆IEC) to identify a transcriptional program downstream of p53 that drives the tumorigenic in-vivo phenotype.
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| |
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| Overall design |
Examination of Differentially Expressed Genes between small intestinal organoids derived from healthy tissue of 8-12 weeks old mice (n = 3) of indicated genotypes.
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| Contributor(s) |
Welz L, Aden K, Rosenstiel P, Mishra N |
| Citation(s) |
34599932 |
| |
| Submission date |
Aug 18, 2021 |
| Last update date |
Dec 08, 2021 |
| Contact name |
Neha Mishra |
| E-mail(s) |
n.mishra@ikmb.uni-kiel.de
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| Organization name |
Institute of Clinical Molecular Biology
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| Lab |
Cell biology Lab
|
| Street address |
Rosalind-Franklin-Str. 12
|
| City |
Kiel |
| ZIP/Postal code |
24105 |
| Country |
Germany |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA755990 |
| SRA |
SRP333084 |
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