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| Status |
Public on Aug 25, 2021 |
| Title |
Single-cell sequencing reveals differential cell types in skin tissues of Liaoning Cashmere Goats and key genes related potentially to the fineness of cashmere fiber |
| Organism |
Capra hircus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
we used single-cell RNA sequencing (scRNA-seq) and computational models to identify 13 skin cell types in Liaoning Cashmere Goats. We also analyzed the molecular changes by Cell Trajectory Analysis in the development process and revealed the maturation process in gene expression profile in Liaoning Cashmere Goats. Weighted gene co-expression network analysis (WGCNA) explored hub genes in cell clusters related to cashmere formation. Secondary hair follicle dermal papilla cells (SDPCs) play an important role in the growth and density of cashmere. ACTA2, a marker gene of SDPCs, was selected for immunofluorescence (IF) and western blot (WB) verification. Our results indicate that ACTA2 is mainly expressed in SDPCs, and WB results showed different expression levels. COL1A1 is a highly expressed gene in SDPCs, which was verified by IF and WB. We then selected CXCL8 of SDPCs to verify, and prove the differential expression in the coarse type and the fine type of Liaoning Cashmere Goats. Therefore, CXCL8 gene may regulate cashmere fineness. These genes may be involved in regulating the fineness of cashmere in goat secondary hair follicle dermal papilla cells, our research will provide new insights into the mechanism of cashmere growth and cashmere fineness regulation by cells.
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| Overall design |
We used single-cell RNA sequencing (scRNA-seq) and computational models to identify 13 skin cell types in Liaoning Cashmere Goats.
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| Contributor(s) |
WANG Z, ZHENG Y, WANG Y, HUI T, CHEN R |
| Citation(s) |
34858469 |
| |
| Submission date |
Aug 19, 2021 |
| Last update date |
Dec 14, 2021 |
| Contact name |
Ying Ze Wang |
| E-mail(s) |
wangzeying2012@syau.edu.cn
|
| Phone |
15998362195
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| Organization name |
沈阳农业大学
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| Street address |
120 Dongling Road, Shenhe District, Shenyang
|
| City |
Shenyang |
| State/province |
Liaoning |
| ZIP/Postal code |
110866 |
| Country |
China |
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| Platforms (1) |
| GPL27106 |
Illumina NovaSeq 6000 (Capra hircus) |
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| Samples (2) |
| GSM5529919 |
The skin tissue of cashmere is taken from scapularis of healthy Liaoning cashmere goats 1 |
| GSM5529920 |
The skin tissue of cashmere is taken from scapularis of healthy Liaoning cashmere goats 2 |
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| Relations |
| BioProject |
PRJNA756314 |
| SRA |
SRP333380 |
