|
| Status |
Public on Jul 25, 2022 |
| Title |
TRIM37 augments AP-2γ transcriptional activity and cellular localization via K63-linked polyubiquitination to drive breast cancer progression [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Using a proteomics approach, we identified the Tripartite Motif Containing 37 (TRIM37) as a novel transcriptional coactivator of AP-2γ. We demonstrate TRIM37 facilitates AP-2γ chromatin binding to regulate the AP-2γ mediated transcriptional program directly. We provide evidence that TRIM37 achieves this by stimulating K63-chain-linked polyubiquitination of AP-2γ, promoting protein localization from the cytoplasm to the nucleus. In clinical analyses, we find TRIM37 is upregulated in multiple breast cancer datasets, supporting our findings that TRIM37-AP-2γ interaction is essential for breast cancer tumor growth. Overall, our work revealed that TRIM37 is an oncogenic coactivator of AP-2γ in breast cancer and provides a novel therapeutic target for treating the disease.
|
| |
|
| Overall design |
ChIP-seq for TRIM37 and AP-2γ in MCF-7 cells
|
| |
|
| Contributor(s) |
Cheung E, Cui G, Narwade N |
| Citation(s) |
35864973 |
| |
| Submission date |
Aug 22, 2021 |
| Last update date |
Jul 29, 2022 |
| Contact name |
Edwin Chong Wing CHEUNG |
| E-mail(s) |
ECheung@um.edu.mo
|
| Organization name |
University of Macau
|
| Department |
Faculty of Health Sciences
|
| Lab |
Edwin CHEUNG's lab
|
| Street address |
Avenida da Universidade, Taipa
|
| City |
Macau |
| State/province |
Macau |
| ZIP/Postal code |
999078 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (5)
|
|
| This SubSeries is part of SuperSeries: |
| GSE182547 |
TRIM37 augments AP-2γ transcriptional activity and cellular localization via K63-linked polyubiquitination to drive breast cancer progression. |
|
| Relations |
| BioProject |
PRJNA756731 |
| SRA |
SRP333585 |
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