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Series GSE182641 Query DataSets for GSE182641
Status Public on Mar 28, 2022
Title Transcriptome analysis of LPS-stimulated BMDMs pretreated with Ctrl MO or Regnase-1-targeting MOs (Reg1-MOs)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Regnase-1 plays essential roles in restricting inflammation by acting as a RNase degrading mRNAs involved in immune reactions via the recognition of stem-loop structures in the 3’untranslated regions (UTRs). Dysregulated expression of Regnase-1 is implicated in the pathogenesis of inflammatory and autoimmune diseases in mice and humans. Here we developed a novel therapeutic strategy to suppress inflammatory responses by blocking Regnase-1 self-regulation, which was enabled by the simultaneous use of two antisense phosphorodiamidate morpholino oligonucleotides (MOs) to alter the binding affinity of Regnase-1 towards the stem-loop structures present in its 3’UTR. The Regnase-1-targeting MOs successfully stabilized Regnase-1 mRNA expression. Furthermore, increasing the abundance of Regnase-1 by MO treatment effectively reduced multiple pro-inflammatory transcripts that were controlled by Regnase-1 in BMDMs. Collectively, these data suggested that MO-mediated disruption of the Regnase-1 self-regulation pathway is an attractive therapeutic strategy to enhance Regnase-1 abundance, which could provide clinical benefits for treating inflammatory diseases through the suppression of inflammation.
 
Overall design Bome marrow cells were isolated from wild-type B6 animals, and were cultured in macrophage growth medium for 7 days (exchange with fresh macrophage growth medium on D4). BMDMs were transfected with Ctrl MO (2 µM) or Reg1-MOs (2 µM) together with Endoporter for 24 hours, followed by stimulation with LPS for 4 hours.
 
Contributor(s) Tse K, Yoshinaga M, Hia F, Takeuchi O
Citation(s) 35544597
Submission date Aug 24, 2021
Last update date Jul 10, 2022
Contact name Ka Man Carman Tse
E-mail(s) isabeltse711@gmail.com
Phone 075-753-9500
Organization name Kyoto University
Department Graduate School of Medicine, Department of Medical Chemistry
Lab Takeuchi lab
Street address Yoshidakonoecho, Sakyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 606-8501
Country Japan
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM5534060 BMDM_Ctrl_MO_LPS_rep1
GSM5534061 BMDM_Ctrl_MO_LPS_rep2
GSM5534062 BMDM_Reg1_MO_LPS_rep1
Relations
BioProject PRJNA757257
SRA SRP333858

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE182641_NormalizedCountData.csv.gz 346.0 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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