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| Status |
Public on Aug 28, 2021 |
| Title |
Inappropriate use of antibiotics exacerbates inflammation through OMVs-induced pyroptosis in multidrug-resistant Klebsiella pneumoniae infection |
| Organism |
Klebsiella pneumoniae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The inappropriate use of antibiotics is a severe public health problem worldwide, contributing to the emergence of multidrug-resistant (MDR) bacteria. To explore the possible impacts of the inappropriate use of antibiotics on the immune system, we use Klebsiella pneumoniae (K. pneumoniae) infection as an example and show that imipenem increases the mortality of mice infected by MDR K. pneumoniae. Further studies demonstrate that imipenem enhances the secretion of outer membrane vesicles (OMVs) with significantly elevated presentation of GroEL, which promotes the phagocytosis of OMVs by macrophages that depends on the interaction between GroEL and its receptor LOX-1. OMVs cause the pyroptosis of macrophages and the release of proinflammatory cytokines, which contribute to exacerbated inflammatory responses. We propose that the inappropriate use of antibiotics in the cases of infection by MDR bacteria such as K. pneumoniae might cause damaging inflammatory responses, which underlines the pernicious effects of inappropriate use of antibiotic.
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| Overall design |
bacterial transcriptional profile with and without treatment with imipenem of Klebsiella pneumoniae
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| Contributor(s) |
Ma Y, Ye C, Huang W, Li W |
| Citation missing |
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| |
| Submission date |
Aug 25, 2021 |
| Last update date |
Aug 28, 2021 |
| Contact name |
Yanbing Ma |
| E-mail(s) |
MolecularImmunology@imbcams.com.cn
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| Phone |
+86 18768156085
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| Organization name |
Chinese Academy of Medical Sciences & Peking Union Medical College
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| Street address |
NO.935 Jiaoling Road
|
| City |
Kunming |
| State/province |
Yunnan |
| ZIP/Postal code |
650106 |
| Country |
China |
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| Platforms (1) |
| GPL28669 |
Illumina NovaSeq 6000 (Klebsiella pneumoniae) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA757732 |
| SRA |
SRP334092 |
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