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Status |
Public on Aug 29, 2021 |
Title |
Analysis of Transcriptome in small intestine after radiation injury with treatment of pravastatin |
Organism |
Sus scrofa domesticus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
This study is designed to understand whether Pravastatin (Pra) treatment alters the transcriptome of small intestine after radiation injury. RNA was extracted from 15 Gy abdominal irradiated Gottingen minipigs with PS treatment. A cDNA library was generated using a TruSeq Stranded mRNA sample prep kit (Illumina, San Diego, CA), and transcriptome sequencing was performed using a TruSeq 3000/4000 SBS kit and Illumina sequencer with 101 bp paired-end reads per sample (Macrogen, Seoul, Korea).
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Overall design |
mRNA profiles of Pra-treated irradiated intestine on Gottingen minipigs were sequenced using Illumina. (2 minipigs of control group, 2 minipigs of irradiation (IR), and 2 minipigs of PS-treated with irradiation (IR+PS))
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Contributor(s) |
Hyosun J, Sehwan S |
Citation(s) |
34688032 |
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Submission date |
Aug 26, 2021 |
Last update date |
Nov 10, 2021 |
Contact name |
Hyosun Jang |
E-mail(s) |
hyosun327@gmail.com
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Phone |
+8229701302
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Organization name |
Korea Institute of Radiological & Medical Science
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Street address |
Nowongu Nowonro 75
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City |
Seoul |
ZIP/Postal code |
01812 |
Country |
South Korea |
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Platforms (1) |
GPL29562 |
Illumina NovaSeq 6000 (Sus scrofa domesticus) |
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Samples (6)
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Relations |
BioProject |
PRJNA758023 |
SRA |
SRP334210 |
Supplementary file |
Size |
Download |
File type/resource |
GSE182829_Expression_Profile.GCF_000003025.6_Sscrofa11.1.gene.txt.gz |
1.9 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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