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| Status |
Public on Sep 30, 2009 |
| Title |
Mild food restriction and gene expression profile (liver) |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
The aim of this study is to obtain the gene expression profiles of the liver of young growing rats after mild restriction of food intake for one week or one month.
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| |
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| Overall design |
Male Wistar rats, at 6 weeks of age, were fed with reduced diet (5%, 10%, 20% and 30% restriction) for one week or one month (n=5). After 16h fasting, the liver was then extracted and total RNA was isolated using an RNeasy mini kit with DNase treatment (QIAGEN). The RNA samples were pooled from five rats for each group. Then, DNA microarray analysis was performed using duplicate GeneChips (Affymetrix) on each pooled RNA sample.
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| Web link |
http://nutrigenomics.jp
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| |
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| Contributor(s) |
Saito K, Ohta Y, Sami M, Kanda T, Kato H |
| Citation(s) |
21256116, 34209243 |
| Submission date |
Sep 27, 2009 |
| Last update date |
Aug 03, 2021 |
| Contact name |
Kenji Saito |
| E-mail(s) |
kkkj774@yahoo.co.jp
|
| Phone |
+81-3-5841-5114
|
| Organization name |
The Univeristy of Tokyo
|
| Department |
Department of Applied Biological Chemistry
|
| Lab |
Nutritional Biochemistry
|
| Street address |
1-1-1, yayoi
|
| City |
bunkyo-ku |
| ZIP/Postal code |
113-8657 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL341 |
[RAE230A] Affymetrix Rat Expression 230A Array |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA118023 |
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