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| Status |
Public on Aug 04, 2022 |
| Title |
Analysis of a neuroblastoma cell line transcriptome by polyA+ RNA sequencing under silencing of RNA helicases DDX5 and DDX17 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
DEAD-box RNA helicases DDX5 and DDX17 regulate gene expression at different levels such as transcription and splicing, but the underlying mechanisms are not fully understood. A transcriptome analysis of DDX5/DDX17-depleted human cells confirmed was carried out to further characterize the widespread deregulation of alternative splicing. This study also revealed a deregulation of 3’ end processing leading to transcriptional read-through.
Univ Lyon, ENS de Lyon, CNRS UMR 5239, INSERM U1293, Laboratory of Biology and Modelling of the Cell, 46 Allée d'Italie, F-69007, Lyon, France.
Corresponding author: cyril.bourgeois@inserm.fr
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| Overall design |
mRNA profiles of DDX5/17-depleted neuroblastoma cell line were generated by sequencing, in triplicate
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| Contributor(s) |
BOURGEOIS CF, TERRONE S, VALAT J, POLVECHE H |
| Citation(s) |
36039747 |
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| Submission date |
Sep 01, 2021 |
| Last update date |
Sep 23, 2022 |
| Contact name |
Hélène Polvèche |
| E-mail(s) |
hpolveche@istem.fr
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| Organization name |
I-Stem
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| Street address |
28, Rue Henri Desbruères
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| City |
Corbeil-Essonnes |
| ZIP/Postal code |
91100 |
| Country |
France |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA759612 |
| SRA |
SRP335223 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE183205_condition_5Y_siDDX5-17_5Y_siGL2.csv.gz |
918.6 Kb |
(ftp)(http) |
CSV |
| GSE183205_rawcounts_siDDX5-17_siGL2.csv.gz |
300.3 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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