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Series GSE18335 Query DataSets for GSE18335
Status Public on Oct 02, 2009
Title Transcriptional changes in the secretome of S. mansoni during early development and in the presence of erythrocytes
Platform organisms Schistosoma japonicum; Schistosoma mansoni
Sample organism Schistosoma mansoni
Experiment type Expression profiling by array
Summary We have examined the gene expression profile of the transformation of the blood fluke Schistosoma mansoni from the cercarial to schistosomula stages. Specifically cercariae were mechanically transformed and then cultured for 3 hours or 5 days, and then total RNA was isolated and microarray analysis performed. A second experiment compares the effect that the presence of red blood cell in the culturing conditions has over the 3 hour and 5 day periods have on the parasite.
 
Overall design Agilent custom designed microarray was used. One colour experimental design. One biological replicate was labelled and then two technical replicates (hybridisations) performed for each sample. A total of 5 samples were examined, cercariae, 3 hrs +RBCs, 5 days + RBCs, 3 hrs –RBCs and 5 days – RBCs.
 
Contributor(s) Gobert GN, Tran MH, Moertel LP, Jones MK, McManus DP, Loukas A
Citation(s) 20161728
Submission date Sep 29, 2009
Last update date Dec 12, 2012
Contact name Alex P Loukas
Phone +61733620222
Organization name QIMR
Street address 300 Herston Road
City Herston
State/province QLD
ZIP/Postal code 4053
Country Australia
 
Platforms (1)
GPL7160 Agilent-016061 QIMR Schistosoma 44k v 1.0
Samples (10)
GSM434496 S. mansoni schistosomula batch 1, 5d replicate 1 of 2
GSM434497 S. mansoni schistosomula batch 1, 5d + RBC replicate 1 of 2
GSM434498 S. mansoni schistosomula batch 1, 3h replicate 1 of 2
Relations
BioProject PRJNA117999

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Supplementary data files not provided
Processed data included within Sample table

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