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Status |
Public on Oct 02, 2009 |
Title |
Transcriptional changes in the secretome of S. mansoni during early development and in the presence of erythrocytes |
Platform organisms |
Schistosoma japonicum; Schistosoma mansoni |
Sample organism |
Schistosoma mansoni |
Experiment type |
Expression profiling by array
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Summary |
We have examined the gene expression profile of the transformation of the blood fluke Schistosoma mansoni from the cercarial to schistosomula stages. Specifically cercariae were mechanically transformed and then cultured for 3 hours or 5 days, and then total RNA was isolated and microarray analysis performed. A second experiment compares the effect that the presence of red blood cell in the culturing conditions has over the 3 hour and 5 day periods have on the parasite.
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Overall design |
Agilent custom designed microarray was used. One colour experimental design. One biological replicate was labelled and then two technical replicates (hybridisations) performed for each sample. A total of 5 samples were examined, cercariae, 3 hrs +RBCs, 5 days + RBCs, 3 hrs –RBCs and 5 days – RBCs.
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Contributor(s) |
Gobert GN, Tran MH, Moertel LP, Jones MK, McManus DP, Loukas A |
Citation(s) |
20161728 |
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Submission date |
Sep 29, 2009 |
Last update date |
Dec 12, 2012 |
Contact name |
Alex P Loukas |
Phone |
+61733620222
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Organization name |
QIMR
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Street address |
300 Herston Road
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City |
Herston |
State/province |
QLD |
ZIP/Postal code |
4053 |
Country |
Australia |
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Platforms (1) |
GPL7160 |
Agilent-016061 QIMR Schistosoma 44k v 1.0 |
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Samples (10)
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GSM434496 |
S. mansoni schistosomula batch 1, 5d replicate 1 of 2 |
GSM434497 |
S. mansoni schistosomula batch 1, 5d + RBC replicate 1 of 2 |
GSM434498 |
S. mansoni schistosomula batch 1, 3h replicate 1 of 2 |
GSM435836 |
S. mansoni schistosomula batch 1, 5d replicate 2 of 2 |
GSM435837 |
S. mansoni schistosomula batch 1, 5d + RBC replicate 2 of 2 |
GSM435838 |
S. mansoni schistosomula batch 1, 3h replicate 2 of 2 |
GSM435839 |
S. mansoni schistosomula batch 1, 3h + RBC replicate 1 of 2 |
GSM435840 |
S. mansoni schistosomula batch 1, 3h + RBC replicate 2 of 2 |
GSM435841 |
S. mansoni cercariae replicate 1 of 2 |
GSM435842 |
S. mansoni cercariae replicate 2 of 2 |
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Relations |
BioProject |
PRJNA117999 |
Supplementary data files not provided |
Processed data included within Sample table |
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